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当前位置: 首页 > 文章 > 聚球藻、盐藻荧光特性研究 安徽农业科学 2010,38 (17) 16-17+24
Position: Home > Articles > Research on the Fluorescence Characteristics of Synechococcus sp.and Salt Algae Journal of Anhui Agricultural Sciences 2010,38 (17) 16-17+24

聚球藻、盐藻荧光特性研究

作  者：
蔡尽忠;庄峙厦;谢增鸿;王小如
单  位：
福州大学化学化工学院;国家海洋局第一海洋研究所;厦门华厦职业学院食品药品安全检测实验室;厦门大学化工学院
关键词：
聚球藻;盐藻;荧光强度;激发光谱;发射光谱
摘  要：
[目的]研究聚球藻和盐藻的荧光特性,确定其在特征激发、发射波长下藻浓度和荧光强度的关系,寻找一种确定藻类浓度的简便方法。[方法]以岛津RF5301型荧光分光光度计分析检测聚球藻和盐藻的激发和发射光谱,并获得它们的荧光强度与以血球计数板计数的藻浓度之间的关系。[结果]聚球藻和盐藻的激发、发射光谱有着明显的不同。在它们特征的激发、发射波长下,其浓度与荧光强度呈良好的线性关系。[结论]通过测定藻类的荧光强度就可推算出特定藻类的浓度,且该方法比血球计数板计算藻类的浓度更为便捷。
译  名：
Research on the Fluorescence Characteristics of Synechococcus sp.and Salt Algae
作  者：
CAI Jin-zhong et al(Laboratory of Food and Drug Safety Test,Xiamen Huaxia Vocational College,Xiamen,Fujian 361024)
关键词：
Synechococcus sp.;Salt algae;Fluorescence intensity;Excitation spectra;Emission spectra
摘  要：
[Objective] A simple determining method of the concentration of the known algae:Synechococcus sp.and salt algae,was formed through the confirmation of the relationship between its concentration and fluorescence intensity under its characteristics of excitation and emission wavelength based on research on its fluorescence characteristics.[Method] The excitation and emission spectra of Synechococcus sp.and salt algae was measured with the fluorescence spectrophotometer(RF5301) and the relationship between the fluorescence intensity and the concentration of alga based on the number at blood counting plate was gotten.[Results] There was a marked difference in the excitation and emission spectra between Synechococcus sp.and salt algae and there was good linear relationship between its concentration and the fluorescence intensity under its special excitation and emission wavelength.[Conclusion] The concentration of specific algae could be calculated through the determination of its fluorescence intensity,and the method was more convenient in the concentration-testing of algae than that of the blood cell counting sheet.

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