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当前位置: 首页 > 文章 > 应用生物素-链霉亲和素的酶联免疫吸附法检测谷物中的玉米赤霉烯酮 安徽农业科学 2009,37 (15) 43-45
Position: Home > Articles > Detection on Zearalenone in Grains by Biotin-streptavidin Enzyme-linked Immunosorbent Assay Journal of Anhui Agricultural Sciences 2009,37 (15) 43-45

应用生物素-链霉亲和素的酶联免疫吸附法检测谷物中的玉米赤霉烯酮

作  者：
马智鸿;黄飚;屠蔷;高蕾;张珏;王柯
单  位：
江苏省原子医学研究所;江南大学生物工程学院生物制药系
关键词：
玉米赤霉烯酮(ZEN);酶联免疫吸附法(ELISA);真菌毒素
摘  要：
基于生物素-链霉亲和素的酶联免疫吸附法(ELISA),建立了玉米赤霉烯酮(ZEN)的ZEN-ELISA检测方法。该方法的检测灵敏度为0.8 ng/ml,批内、批间变异系数分别为5.1%和8.6%,平均加样回收率为91.2%;抗ZEN单克隆抗体与玉米赤霉醇的交叉反应度为12.3%,而其与赭曲霉素A、脱氧雪腐镰刀菌烯醇、黄曲霉素B1无交叉反应;相关试剂在4℃存180 d后各检测参数基本无变化;该方法与商品ELISA试剂盒对同一样品ZEN测定结果的相关系数为0.943 2。说明该方法的特异性和稳定性较好,测定结果准确,可用于ZEN的大量筛查。
译  名：
Detection on Zearalenone in Grains by Biotin-streptavidin Enzyme-linked Immunosorbent Assay
作  者：
MA Zhi-hong et al(Jiangsu Institute of Molecular Medicine,Key Laboratory of nuclear medicine of Health Ministry,Wuxi,Jiangsu 214063)
关键词：
Zearalenone(ZEN);Enzyme-linked immunosorbent assay(ELISA);Mycotoxins
摘  要：
Based on biotin-streptavidin enzyme-linked immunosorbent assay(ELISA),the detection method on zearalenone ZEN-ELISA was established.The detection sensitivity of this method was 0.8 ng/ml,and the variation coefficients of intra-assay and inter-assay were 5.1% and 8.6% resp.,and the average recovery was 91.2%.The cross reaction rate of monoclonal antibody against ZEN and zearalanol was 12.3%,and there were no reaction between monoclonal antibody against ZEN and ochratoxin A,deoxynivalenol,aflatoxin B1.When related reagents were stored at 4 ℃ for 180 d,the detection parameters almost invariant. The correlation coefficient of the detection results of ZEN-ELISA method and commercial ELISA kit on the same sample was 0.943 2.Which indicated that the specific and stability of ZEN-ELISA method were better,and its detection results was accurate,so it could be used to largely screen ZEN.

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