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当前位置: 首页 > 文章 > 金橘皮中黄酮类物质的提取及其体外抗氧化活性研究 农业工程学报 2008,24 (6) 258-261
Position: Home > Articles > Extraction of flavone compounds from Fortunella margarita peel and its antioxidation in vitro Transactions of the Chinese Society of Agricultural Engineering 2008,24 (6) 258-261

金橘皮中黄酮类物质的提取及其体外抗氧化活性研究

作  者：
唐巧玉;周毅峰;朱玉昌;云鑫
单  位：
湖北民族学院生物资源保护与利用省重点实验室;湖北民族学院生物科学与技术学院
关键词：
金橘;黄酮;提取;抗氧化
摘  要：
为了确定金橘皮中黄酮类物质的提取工艺和初步鉴定该类物质的活性,该文以黄酮得率为考察指标,比较了不同溶剂对金橘皮黄酮类物质的提取效果,并采用正交试验,研究了提取溶剂浓度、提取温度、时间和料液比对金橘皮黄酮类化合物提取的影响。同时,以粗黄酮类物质的还原能力、对超氧阴离子自由基(O-2·)、羟基自由基(·OH)的清除率作为考查指标,研究了金橘皮黄酮类物质的体外抗氧化活性。确定了最佳提取工艺条件为:最佳提取溶剂为甲醇,甲醇浓度为60%,料液比为1:40,温度80℃,提取时间4h。粗黄酮具有较强的还原能力,高于同浓度下的抗氧化剂2,6-二叔丁基对甲酚(BHT)和没食子酸丙酯(PG);对O-2·、·OH均具有良好的清除作用,但对O-2·的清除效果更好。
译  名：
Extraction of flavone compounds from Fortunella margarita peel and its antioxidation in vitro
作  者：
Tang Qiaoyu1, Zhou Yifeng2, Zhu Yuchang1, Yun Xin2 (1. Key Laboratory of Biological Resources Protection and Utilization of Hubei Province, Enshi 445000, China; 2. Department of Biological Science and Technology of Hubei Institute for Nationalities, Enshi 445000, China)
关键词：
Fortunella margarita; flavones; extraction; antioxidation
摘  要：
In order to determine extraction process of flavones from Fortunella margarita peel and authenticate the activity of the flavones, the extraction efficiency of different solvents for flavones in Fortunella margarita peel was compared and the influences of various parameters such as solvent concentration, temperature, time or the ratio of Fortunella margarita to solvent on extraction efficiency by use of orthogonal experiment were studied taking yield of flavones as study index. At the same time, taking reducing ability, clearance ability forO2-· and ·OH as study index, its antioxidation in vitro was studied. The optimized parameters were as follows: methanol was used as extraction solvent, methanol concentration was 60%, the ratio of Fortunella margarita to methanol was 1︰40, the temperature was 40℃ , and the extraction time was 2 h. The raw flavones had better reducing ability that was better than antioxidant such as 2,6- ditert-butyl-4-methyl phenol (BHT) or propyl gallate (PG) under the same concentration. At the same time, it had strong clearance ability forO2-· and ·OH, but the effect onO-2· was better than that of ·OH.

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