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当前位置: 首页 > 文章 > 粗山羊草新型硬度等位基因的克隆及分析 麦类作物学报 2015,35 (9) 1202-1207
Position: Home > Articles > Molecular Cloning and Sequence Analysis of the Novel Puroindoline Genes fromAegilops tauschii Journal of Triticeae Crops 2015,35 (9) 1202-1207

粗山羊草新型硬度等位基因的克隆及分析

作  者：
曹雯梅;刘述忠;张亚菲;郑明辉
单  位：
河南农业职业学院;河南大学生命科学学院
关键词：
籽粒硬度;粗山羊草;等位基因;基因克隆;序列分析
摘  要：
小麦籽粒硬度是其最为重要的品质性状之一,现已发现控制小麦籽粒硬度的主效基因是Pina和Pinb。为了丰富籽粒硬度新型等位基因,根据已报道的小麦Pina和Pinb基因的保守序列,设计了2对特异性引物,对粗山羊草(Aegilops tauschii)进行Pina和Pinb基因的PCR扩增、克隆及序列分析。结果表明,本研究共得到2个新型Pina等位基因和3个新型Pinb等位基因,核酸序列长度均为447bp,编码148个氨基酸残基,具有麦类作物PINA和PINB蛋白所特有的色氨酸结构域:WRWWKWWK和WPTKWWK。与已知的Pina和Pinb基因序列相比较,这些基因含有多个SNP变异位点,丰富了小麦籽粒硬度改良的遗传资源。
译  名：
Molecular Cloning and Sequence Analysis of the Novel Puroindoline Genes fromAegilops tauschii
作  者：
CAO Wenmei;LIU Shuzhong;ZHANG Yafei;ZHENG Minghui;Henan Vocational College of Agriculture;College of Life Science,Henan University;
关键词：
Kernel hardness;;Aegilops tauschii;;Puroindoline alleles;;Gene cloning;;Sequence analysis
摘  要：
Grain hardness is one of the main quality characters of wheat.It has been revealed that the major genes controlling grain hardness in wheat were Pinaand Pinb.According to the conserved regions of Pinaand Pinb genes reported in wheat,two pairs of specific primers were designed and used to amplify in wheat D-genome progenitor,Aegilops tauschii.The results showed that two novel Pina alleles and three Pinballeles were obtained.Sequence analysis indicated that all of two Pinagenes and three Pinb genes were 447 bp in size,encoding 148 amino acid residues containing the tryptophan motif of WRWWKWWK and WPTKWWK,which were specific to PINA and PINB in cereal crop.Compared with the reported Pina and Pinb sequences,some SNPs were also identified in the Pina and Pinb alleles of Ae.tauschii.The result enriched genetic resources for kernel hardness improvement of wheat.

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