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当前位置: 首页 > 文章 > 利用基因工程抗体建立的新型双抗夹心ELISA法检测Cry1Ac毒素 江苏农业学报 2014 (3) 634-639
Position: Home > Articles > Establishment of a new double-antibody sandwich ELISA method for detection of Cry1Ac toxin with genetically engineered antibody Jiangsu Journal of Agricultural Sciences 2014 (3) 634-639

利用基因工程抗体建立的新型双抗夹心ELISA法检测Cry1Ac毒素

作  者：
张留圈;张霄;刘媛;徐重新;张存政;谢雅晶;颜春荣;寇莉萍;刘贤金
单  位：
江苏省农业科学院食品质量安全与检测研究所;江苏省食品质量安全重点实验室-省部共建国家重点实验室培育基地;西北农林科技大学食品科学与工程学院;农业部农产品质量安全控制技术与标准重点实验室;江苏省产品质量监督检验研究院
关键词：
基因工程抗体;双抗夹心ELISA;Cry1Ac毒素
摘  要：
为明确新型双抗夹心ELISA方法检测Cry1Ac毒素的效果,分别用纯化后的抗Cry1Ac单链抗体和anti-Cry1Ac兔多克隆血清(Anti-Cry1Ac-PAbs)作为捕获抗体和检测抗体,通过方阵滴定确定其最佳工作浓度,建立双抗夹心ELISA法。再用优化后的双抗夹心ELISA方法对检测Cry1Ac毒素的敏感性、特异性和回收率进行鉴定。结果表明,新型双抗夹心ELISA法最低检测限为0.91 ng/ml,线性检测范围为1.04 ng/ml~3.49μg/ml。5种稻米中Cry1Ac毒素的平均回收率为69.42%~87.95%,变异系数为1.19%~6.50%。
译  名：
Establishment of a new double-antibody sandwich ELISA method for detection of Cry1Ac toxin with genetically engineered antibody
作  者：
ZHANG Liu-quan;ZHANG Xiao;LIU Yuan;XU Chong-xin;ZHANG Cun-zheng;XIE Ya-jing;YAN Chun-rong;KOU Li-ping;LIU Xian-jin;College of Food Science and Engineering,Northwest A&F University;Key lab of Food Quality of Jiangsu Province-State Key Laboratory Breeding Base /Key Laboratory of Control Technology and Standard for Agro-product Safety and Quality,Ministry of Agriculture /Institute of Food Safety,Jiangsu Academy of Agricultural Sciences;Jiangsu Product Quality Testing and Inspection Institute;
关键词：
genetically engineered antibody;;double-antibody sandwich ELISA;;Cry1Ac toxin
摘  要：
The purified anti-Cry1Ac single chain antibody was used as capture antibody,and genetically engineered anti-Cry1Ac-PAbs was used as detection antibody to develop a double-antibody sandwich ELISA for detection of Cry1Ac toxin. The results showed that the limit of detection of Cry1Ac toxin was 0. 91 ng /ml,with the linear range from1. 04 to 3. 49 ng /ml. The average recovery of Cry1Ac toxin in five kinds of rice was 69. 42%- 87. 95%,with coefficient of variation of 1. 19%- 6. 50%.

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