Optimization of Supercritical Carbon Dioxide Extraction of Oil from Sloanea hemsleyana Seeds Using Response Surface Methodology

MA Cheng-jin1,2,WU Zhu-qing2,FU Wei-chang1,2,HUANG Qun1,2,CHEN Gong-xi1(1.Key Laboratory of Plant Resources Conservation and Utilization of Hunan Province,Jishou University,Jishou 416000,China;2.Institute of Food Science,Jishou University,Jishou 416000,China)

Sloanea hemsleyana seed oil;supercritical carbon dioxide extraction;technological conditions;response surface analysis;GC-MS

Response surface methodology(RSM) was applied to optimize the extraction conditions of oil from Sloanea hemsleyana seeds.According to single factor investigations,extraction pressure,temperature and duration and separation temperature were the most important factors affecting oil yield,and carbon dioxide flow rate of 25 kg/h and material particle size of 40 mesh were optimal for oil extraction from Sloanea hemsleyana seeds.A mathematical model with oil yield as a response to extraction pressure,temperature and duration and separation temperature was established using Box-Behnken central composite experimental design.This was followed by response surface analysis.It was found that the optimal process conditions for oil extraction from Sloanea hemsleyana seeds were as follows:extraction pressure 31 MPa;extraction temperature 47℃;separation temperature 34℃;and extraction duration 72 min and that the resultant oil yield was 48.57%.Meanwhile,GC-MS analysis of fatty acid composition of the extracted oil was conducted,and the results showed that the oil was abundant in unsaturated fatty acids contained 35.17% of oleic acid and 19.76% of linoleic acid.