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当前位置: 首页 > 文章 > 2007年华北地区H3N8亚型马流感病毒的分离与鉴定 中国兽医学报 2010,30 (5) 607-611
Position: Home > Articles > Isolation and Identification of Equine Influenza Virus Subtype H3N8 causing an outbreak in Northern China in 2007 Chinese Journal of Veterinary Science 2010,30 (5) 607-611

2007年华北地区H3N8亚型马流感病毒的分离与鉴定

作  者：
蒋桃珍;刘月焕;林健;韩春华;潘洁;赖平安;韦海涛;祝俊杰;赵景义;马志军;毕丁仁
单  位：
华中农业大学动物医学院;北京市农林科学院畜牧兽医研究所;北京市畜牧兽医总站;北京出入境检验检疫局
关键词：
马流感病毒;H3N8;马;分离鉴定
摘  要：
2007年10月,华北地区某赛马场的马同时发生了以发烧、流水样鼻汁或脓性分泌物、咳嗽等临床症状为主的疾病,疑似马流行性感冒。采集患病赛马的鼻腔分泌物,发病期和发病后14d血清,经鸡胚接种法分离病毒,并用鸡红细胞血凝抑制试验(HI)、神经氨酸酶抑制试验(NI)、病毒回归试验、血清学检测和基因序列分析对分离的病毒进行了系统鉴定。结果表明分离的毒株(A/equine/Huabei/1/2007(H3N8)为马源H3N8亚型马流感病毒,基因型属于美洲分支。我们通过动物回归感染试验建立起分离毒株的实验感染模型。
译  名：
Isolation and Identification of Equine Influenza Virus Subtype H3N8 causing an outbreak in Northern China in 2007
作  者：
JIANG Tao-zhen1,5,LIU Yue-huan2,LIN Jian2,HAN Chun-hua2,PAN Jie2,LAI Ping-an3,WEI Hai-tao4,ZHU Jun-jie4,ZHAO Jing-yi4,MA Zhi-jun4,BI Ding-ren1 (1.College of Veterinary Medicine,Huazhong Agricultural University,Key Laboratory of Preventive Medicine of Hubei Province,Wuhan 430070,China;2.Institute of Animal Husbandry and Veterinary Medicine,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;3.Beijing Entry-Exit Inspection and Quarantine Bureau,Beijing 100029,China;4.Beijing Municipal Husbandry and Veterinary General Station,Beijing 100029,China;5.China Institute of Veterinary Drug Control,Beijing 100081,China)
关键词：
Equine influenza;H3N8;horses;isolation and identification
摘  要：
In October 2007,an outbreak of severe respiratory disease with the developing clinical symptoms such as depression,anorexia,dry cough,high fever and mucopurulent nasal discharge occurred in race horses in Northern China.We took the samples of nasal discharge and paired sera and examined the presence of equine influenza virus and autibody.Embryonated SPF eggs were used for virus inoculation from nasopharyngeal swabs,viral growth was monitored by haemagglutination assay (HA) and the isolate was characterized by haemagglutination inhibition (HI) and neuraminidase inhibition (NI) assay.Four Mongolia horses as experimental animals were inoculated with the isolate for pathogenic experiment and the nucleotide of isolate was sequenced for sequence comparison.Serological test was also done for H3N8 subtype HI antibody in the serum samples.Results indicated that an influenza virus,A/equine/Huabei/1/2007(H3N8),was isolated from horses with an acute respiratory infection,phylogenetic analyses of isolate indicated that each of 8 gene sequences are of equine origin and belong to one of the Americans cluster.The data of epidemiology revealed that H3N8 subtype equine influenza occurred in Northern China and experimental model of isolates was established successfully.

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