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当前位置: 首页 > 文章 > 云南蔷薇属部分种质资源的SSR遗传多样性研究 园艺学报 2008,35 (8) 1227-1232
Position: Home > Articles > Study on Genetic Diversity of Some Rosa Germplasm in Yunnan Based on SSR Markers Acta Horticulturae Sinica 2008,35 (8) 1227-1232

云南蔷薇属部分种质资源的SSR遗传多样性研究

作  者：
唐开学;邱显钦;张颢;李树发;王其刚;蹇洪英;鄢波;黄兴奇
单  位：
云南大学生命科学学院;云南省农业科学院花卉研究所
关键词：
蔷薇属;遗传多样性;亲缘关系;SSR
摘  要：
利用简单重复序列SSR标记技术对蔷薇属(Rosa L.)13份野生种、变种、变型及29份栽培品种的遗传多样性进行了研究。用筛选出的18对SSR引物对42份材料DNA进行PCR扩增,在18个位点共检测到148个等位基因,每一位点的等位基因变幅为6~14个,平均8.2个。材料间遗传相似系数为0.282~0.892,表明在分子水平上具有丰富的遗传多样性。在相似系数为0.456时,基于SSR标记的聚类分析将13个蔷薇野生种分为5个组,这与植物形态学分类结果大体一致。在遗传相似系数为0.43水平上,聚类分析将42份供试材料分为5大组群。初步探讨了野生种之间以及野生种与栽培品种之间的亲缘关系。
译  名：
Study on Genetic Diversity of Some Rosa Germplasm in Yunnan Based on SSR Markers
作  者：
TANG Kai-xue1,2,QIU Xian-qin2,ZHANG Hao2,LI Shu-fa2,WANG Qi-gang2,JIAN Hong-ying2,YAN Bo2,and HUANG Xing-qi1,3(1College of Life Sciences,Yunnan University,Kunming 650091,China;2Flower Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China;3Yunnan Key Laboratory of Agricultural Biotechnology,Kunming 650223,China)
关键词：
Rosa L.;genetic diversity;genetic relationship;SSR
摘  要：
The genetic diversity of 42 Rosa germplasm including 13 accessions of wild species(varieties,forms)and 29 rose cultivars in Yunnan was studied by simple sequence repeat(SSR).Eighteen pairs of SSR primers were used in PCR amplification with genomic DNA as template.A total of 148 alleles were detected at 18 loci.The number of alleles per locus ranged from 6 to 14,with an average of 8.2.The genetic similar coefficient ranged from 0.282 to 0.892,which showed that the genetic diversity among 42 rose accessions in Yunnan was abundant based on SSR molecular markers.The 13 Rosa species could be divided into five groups obviously based on the UPGMA cluster at the similar coefficient 0.456,which is consensus of botanical morphological classification.All the species and cultivars were divided into five groups at the similar coefficient of 0.43 by UPGMA cluster.Meanwhile genetic relationships between 42 rose accessions were discussed preliminarily.

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