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当前位置: 首页 > 文章 > 绵羊肺腺瘤内、外源性env基因比较分析 中国动物传染病学报 2013 (3) 18-26
Position: Home > Articles > ANALYSIS OF ENDOGENOUS AND EXOGENOUS ENV GENES OF JAAGSIEKTE SHEEP RETROVIRUS Chinese Journal of Animal Infectious Diseases 2013 (3) 18-26

绵羊肺腺瘤内、外源性env基因比较分析

作  者：
孔汉金;张克山;刘永杰;尚佑军;吴斌;刘湘涛
单  位：
中国农业科学院兰州兽医研究所;华中农业大学
关键词：
绵羊肺腺瘤;env基因;序列分析;结构预测
摘  要：
绵羊肺腺瘤(Ovine pulmonary adenocarcinoma,OPA)是由绵羊肺腺瘤反转录病毒(Jaagsiekte retrovirus,JSRV)感染引起的成年绵羊的一种慢性、进行性、接触传染性肺脏疾病。env是JSRV的主要毒力基因之一,而在正常羊的基因组中也存在着JSRV的内源性基因片段。为比较分析JSRV内、外源性env的特征,本文扩增并测定了正常绵羊肺组织的内源性env基因(Enenv),运用生物信息学技术,比较分析了其与外源性绵羊肺腺瘤病毒JSRV21(AF105220)env基因(Exenv)的遗传进化关系,二、三级蛋白结构和抗原表位。结果表明Enenv和Exenv核苷酸同源性为88.1%,推导的氨基酸同源性为92.0%,两者的主要差异位于TM区和YXXM基序。本结果为进一步研究JSRVenv基因功能提供了新线索。
译  名：
ANALYSIS OF ENDOGENOUS AND EXOGENOUS ENV GENES OF JAAGSIEKTE SHEEP RETROVIRUS
作  者：
KONG Han-jin1,2,ZHANG Ke-shan1,LIU Yong-jie1,SHANG You-jun1,Wu-bin2,LIU Xiang-tao1(1.National Foot and-Mouth Disease Reference Laboratory,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,CAAS,Lanzhou 730046,China;2.State Key Laboratory of Agriculture Microbiology,College of Veterinary Medicine,Huazhong agricultural University,Wuhan 430070,China)
关键词：
Ovine pulmonary adenomatosis;env gene;sequences analysis;structure prediction
摘  要：
Ovine pulmonary adenomatosis(OPA) is a chronic,progressive and contagious lung tumor in adult sheep,which is caused by Jaagsiekte sheep retrovirus(JSRV).The envelop gene plays a key role in pathogenesis of OPA.On the other hand,an important finding is that multiple copies of highly related endogenous JSRV-like proviruses have been also identified in normal sheep genome.In order to analyze the difference of envelope genes between endogenous and exogenous Jaagsiekte sheep retroviruses,we amplified and sequenced env gene of enJSRV(Enenv) from lung tissue of normal sheep.The nucleotide and amino acid sequences of env gene were compared with JSRV21 strain(AF105220)(Exenv) published on the GenBank using DNAstar software.Then we predicted and compared the secondary structure,tertiary structure and antigenic epitopes of env genes of enJSRV and exJSRV.The results indicated that Enenv shared 88.1% homology with Exenv at nucleotide level and 92.0% homology at amino acid level.The main difference between them was located in the transmembrane(TM) domains of the envelope protein.The cytoplasmic tail(CT) of TM domain of JSRV21 Exenv possessed a specialYXXM motif,which was absent in Enenv.The present research has provided experimental and theoretical bases for further studying the function of endogenous and exogenous env genes of JSRV.

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