中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 硫酸安普霉素对嗜水气单胞菌体外抑菌和体内抗感染研究 淡水渔业 2012,42 (5) 33-37
Position: Home > Articles > Studies on inhibition of apramycinsulphate on Aeromonas hydrophila in vitroand resistance of Litopenaeus vannameiagainst A.hydrophila in vivo Freshwater Fisheries 2012,42 (5) 33-37

硫酸安普霉素对嗜水气单胞菌体外抑菌和体内抗感染研究

作  者：
孙智武;黄燕华;曹俊明;王国霞;陈冰;莫文艳;周婷婷;刘群芳
单  位：
广东省农业科学院畜牧研究所
关键词：
硫酸安普霉素;嗜水气单胞菌(Aeromonas hydrophila);凡纳滨对虾(Litopenaeus vannamei);抑菌效果
摘  要：
研究了硫酸安普霉素(apramycin sulphate)对嗜水气单胞菌(Aeromonas hydrophila,A.h)的体外抑菌效果及饲料中添加硫酸安普霉素对凡纳滨对虾(Litopenaeus vannamei)体内抗A.h感染能力的影响。结果显示:硫酸安普霉素MIC、MBC和MBC/MIC分别为0.5μg/mL、1.0μg/mL和2;硫酸安普霉素在A.h调整期具有明显的抑菌效果,属于静止期杀菌药物,具有较长的抗菌后效应(PAE),呈明显的浓度依赖性;经过10代继代培养,其耐药性获得速率为2倍。A.h浸泡感染96 h后,对照组累积死亡率为86.7%,添加组为44.3%～66.7%,相对保护率为23.1%～50.1%。结果表明,硫酸安普霉素对A.h具有良好的体外抑菌效果,不易产生耐药性;在饲料中添加硫酸安普霉素,可以提高凡纳滨对虾抗A.h感染能力,添加量为240 mg/kg饲料时作用最显著。
译  名：
Studies on inhibition of apramycinsulphate on Aeromonas hydrophila in vitroand resistance of Litopenaeus vannameiagainst A.hydrophila in vivo
作  者：
SUN Zhi-wu1,2,HUANG Yan-hua1,CAO Jun-ming1,WANG Guo-xia1,CHEN Bing1, MO Wen-yan1,ZHOU Ting-ting1,LIU Qun-fang1 (1.Institute of Animal Science,Guangdong Academy of Agricultural Sciences/Guangdong Public Laboratory of Animal Breeding and Nutrition/Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangzhou510640,China; 2.College of Fisheries,Huazhong Agricultural University,Wuhan430070,China)
关键词：
apramycin surphate;Aeromonas hydrophila;Litopenaeus vannamei;antibacterial activity
摘  要：
This study was conducted to investigate the inhibition of apramycin sulphate on Aeromonas hydrophila in vitro and the effect of dietary apramycin sulphate on resistance of Litopenaeus vannamei against A.hydrophia in vivo.The results showed that MIC,MBC and MBC/MIC were 0.5 μg/mL,1.0 μg/mL and 2,respectively.Apramycin sulphate obviously inhibited the growth of A.hydrophia at lag phase.It was concentration-dependent according to the killing curve and PAE.The maximum drug action of apramycin sulphase appeared at the stationary phase,and the PAE of apramycin sulphate was conspicuous in vitro.The rate of drug resistance obtained in A.hydrophila against apramycin sulphate doubled in the 10 days' subculture.After 96 hours' infection of A.hydrophila,accumulative mortality rate(AMR) in control group was 86.7%,and that in the groups with apramycin sulphate was from 43.3% to 66.7%.The relative percentage survival(RPS) in these groups was from 23.1% to 50.1%.In conclusion,apramycin sulphate was effective on anti-A.hydrophila in vitro,and could improve the resistance of L.vannamei against A.hydrophila in vivo.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 1

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46