中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 新疆嗜菌异小杆线虫及其共生细菌的初步鉴定研究 新疆农业科学 2015 (2) 244-251
Position: Home > Articles > Preliminary Identification of Entomopathogenic Nematodes(Heterorhabditis bacteriophora strain Nileke)and Its Symbiotic Bacteria in Xinjiang Xinjiang Agricultural Sciences 2015 (2) 244-251

新疆嗜菌异小杆线虫及其共生细菌的初步鉴定研究

作  者：
詹发强;布卡欧尔娜;侯敏;杨蓉;龙宣杞;Xuan qi
单  位：
新疆农业大学;Research;新疆农业科学院微生物应用研究所
关键词：
昆虫病原线虫;嗜菌异小杆线虫;共生细菌;分类鉴定
摘  要：
【目的】分离获得新疆地区的昆虫病原线虫及其共生细菌,确定其种属及共生细菌的分类地位,为新疆利用昆虫病原线虫进行生物防治害虫提供新的选择和奠定基础。【方法】采用黄粉甲(Tenebrio molitor)幼虫为诱导昆虫,采用Whitetrap法收集侵染期线虫,通过致死的昆虫血腔和侵染期线虫直接分离共生细菌,利用18S rRNA与形态鉴定初步确定昆虫病原线虫的种属,利用16S rRNA与形态初步确定其共生细菌的分类地位。【结果】从新疆伊犁州尼勒克县一山区牧场林带土壤中分离出一种能够有效致死黄粉甲幼虫的昆虫病原线虫,经初步鉴定其属于异小杆属线虫,与嗜菌异小杆线虫进化距离最近,初步命名为嗜菌异小杆线虫尼勒克品系(Heterorhabditis bacteriophora strain Nileke);分离出其共生细菌,初步鉴定为Photorhabdus luminescens(NLK-1),但无荧光。【结论】在新疆本土成功分离出一种异小杆属昆虫病原线虫及其共生细菌,初步确定了其种属,为在新疆本土开展以昆虫病原线虫为主的生物防治害虫提供了新的选择及途径,为开展本地区更广泛的分离筛选奠定了基础。
译  名：
Preliminary Identification of Entomopathogenic Nematodes(Heterorhabditis bacteriophora strain Nileke)and Its Symbiotic Bacteria in Xinjiang
作  者：
ZHAN Fa-qiang;Buka Ouerna;HOU Min;YANG Rong;LONG;Xuan-qi;Research Institute of Applied Microbiology of Xinjiang Academy of Agricultural Sciences;Xinjiang Agricultural University;
关键词：
entomopathogenic nematodes;;Heterorhabditis bacteriophora;;symbiotic bacteria;;identification
摘  要：
【Objective】To obtain a local entomopathogenic nematodes and their symbiotic bacteria,determine their species and taxonomic status,provide new options and lay the foundation of using our region's entomopathogenic nematodes to control pests in future. 【Method】Using Tenebrio molitor larve to induce entomopathogenic nematodes,adopting the method of whitetrap to collect infective juveniles. Symbiotic bacteria isolated directly through infective juveniles or the blood chamber of Tenebrio molitor larve which were infected by entomopathogenic nematodes,using 18 S rRNA and morphological to identify the species of nematodes initially,16 S rRNA and morphological to identify the species of symbiotic bacteria initially;【Result】One kind of entomopathogenic nematodes was isolated from forest soils in Nileke,Xinjiang which could kill Tenebrio molitorlarve effectively. It belonged to Heterorhabditis nematodes after a preliminary identification of genus,and named as Heterorhabditis bacteriophora strain Nileke initially. While its symbiotic bacteria were isolated and identified as Photorhabdus luminescens,but no fluorescence; 【Conclusion】The entomopathogenic nematodes and its symbiotic bacteria were the first time successfully separated in Xinjiang and its species were initially identified,which might offer new options and ways to control pests in the future by entomopathogenic nematodes isolated from Xinjiang and which also established a good foundation to isolate entomapathogenic nematodes in more places in Xinjiang in the near future.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46