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当前位置: 首页 > 文章 > 华山松大小蠹共生真菌秦岭细粘束孢产纤维素酶的研究 林业科学 2009,45 (10) 108-112
Position: Home > Articles > Cellulase Produced by the Symbiotic Fungus Leptographium qinlingensis of Dendroctonus armandi Scientia Silvae Sinicae 2009,45 (10) 108-112

华山松大小蠹共生真菌秦岭细粘束孢产纤维素酶的研究

作  者：
高瑞霞;陈辉;唐明;徐辉;李守萍;陈桂梅
单  位：
西北农林科技大学林学院;西北农林科技大学生命科学学院
关键词：
华山松大小蠹;秦岭细粘束孢;纤维素酶;正交试验
摘  要：
通过单因素和正交试验对秦岭细粘束孢分泌纤维素酶的组分、条件等进行研究。结果表明:秦岭细粘束孢可以分泌内切葡萄糖苷酶(CX)、外切葡萄糖苷酶(C1)和β-葡萄糖苷酶(BG),具有完整的纤维素酶系;其中CX是秦岭细粘束孢分泌的主要纤维素酶组分,优化后CX酶的最大酶活为35.215U,分别是C1和BG最大酶活的13.662倍和8.780倍。正交试验表明:秦岭细粘束孢分泌CX,C1和BG的最适条件各不相同,产CX的最佳条件为29℃、6天、起始pH4.0、摇床转速150r.min-1。温度、转速、起始pH和时间4个因素对秦岭细粘束孢产3种酶的影响各不相同,影响最大的是温度,其次是转速和时间,而起始pH对该菌产酶的影响最小。
译  名：
Cellulase Produced by the Symbiotic Fungus Leptographium qinlingensis of Dendroctonus armandi
作  者：
Gao Ruixia1 Chen Hui2 Tang Ming2 Xu Hui1 Li Shouping1 Chen Guimei1(1.College of Life Sciences,Northwest A&F University Yangling 712100;2.College of Forestry,Northwest A&F University Yangling 712100)
关键词：
Dendroctonus armandi;Leptographium qinlingensis;cellulase;orthogonal experiment
摘  要：
Components of cellulase produced by Leptographium qinlingensis and the optimum conditions for biosyntheses of the enzymes were studied by single factor and orthogonal experiment in this paper.The result showed that Leptographium qinlingensis had a complete cellulase system which was composed of endo-β-1,4-glucanases(C_X),exo-β-1,4-glucanases(C_1)and β-1,4-glucosidase(BG).Furthermore,it was also showed that the enzyme C_X was the major component of the cellulase whose optimum activity(35.215 U)was 13.662 times higher than enzyme C_1 and 8.780 higher times than enzyme BG respectively.A orthogonal experiment indicated that the optimum conditions of enzyme C_X,enzyme C_1 and enzyme BG were different.The optimum conditions to produce enzyme C_X were at 29 ℃ with six-day cultivating time,pH value 4.0 and 150 r·min-1 rotating speed.Among the four factors(temperature,cultivating time,pH value and rotating speed),temperature,had the largest impact on producing cellulase by Leptographium qinlingensis,followed by cultivation time and rotating speed.

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