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Position: Home > Articles > Effects of Somatostatin Fusion Gene Carried with PLGA Microspheres on Rabbits Growth Chinese Journal of Animal and Veterinary Sciences 2008,39 (10) 1415-1420

PLGA微球包裹生长抑素融合基因表达质粒对家兔生长的影响

作  者:
戴建威;章倩倩;王妍;刘松财;张永亮
单  位:
广州医学院生化教研室;中山大学生物工程中心真核分子生物学实验室;华南农业大学动物科学学院;延边大学农学院动物医学系;吉林大学畜牧兽医学院
关键词:
生长抑素;核酸免疫;乙肝表面抗原;PLGA;免疫中和
摘  要:
将HBsAg与Somatostatin(SS)融合基因克隆到真核表达载体pIRES1neo-CMV,构建了pIRES1neo-HB-sAg/SSM。应用脂质体介导转染CHO细胞,96 h提取细胞总RNA,RT-PCR鉴定融合基因表达;并收集细胞上清,ELISA法测定上清中HBsAg表达情况,结果为阳性。大量提取pIRES1neo-HBsAg/SSM质粒,用Poly D,L-lactide-co-glycolic acid(PLGA)微球包裹,注射Vc獭兔后肢胫前肌,以注射生理盐水獭兔为对照。动物常规饲养45 d,观察日增重并测定血清中IGF-Ⅰ、SS含量以及抗HBsAg抗体水平。结果显示注射后15~30 d,质粒注射组日增重较对照组高106.8%(P<0.01);30 d时,血清中IGF-Ⅰ浓度质粒组比对照组高29.2%(P<0.05);检测整个过程中血清中SS含量,发现注射后30 d时明显低于对照组,且出现整个试验过程的最低值;质粒注射组血清中抗HBsAg抗体水平在注射后30 d时出现峰值,这与SS含量出现最低值相吻合。本试验结果表明HBsAg与SS融合基因免疫獭兔,可以促进动物生长。
译  名:
Effects of Somatostatin Fusion Gene Carried with PLGA Microspheres on Rabbits Growth
作  者:
DAI Jian-wei2,3,ZHANG Qian-qian4,WANG Yan5,LIU Song-cai3,ZHANG Yong-liang1*(1.College of Animal Science,South China Agricultural University,Guangzhou 510642,China;2.Department of Biochemistry,Guangzhou Medical College,Guangzhou 510182,China;3.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China;4.Key Laboratory of Gene Engineering of the Ministry of Education,Biotechnology Research Center,Sun Yat-sen University,Guangzhou 510275;China;5.Veterinary Medicine Department,Agricultural College,Yanbian University,Longjing 133400,China)
关键词:
somatostatin;gene vaccine;HBsAg;PLGA;immuno-neutralization
摘  要:
In this paper,we described the regulation of animal growth using plasmid DNA encoding the SS gene that fused with the hepatitis B surface antigen(HBsAg) gene.The fusion genes were successfully expressed in CHO cells,as proven by RT-PCR and ELISA assayed.Poly D,L-lactide-co-glycolic acid(PLGA) plasmid-encapsulating microspheres were prepared and injected directly into the anterior tibialis of rabbits.Daily weight gain and the levels of insulinlike growth factor-Ⅰ(IGF-Ⅰ),SS,and hepatitis B surface antibody(HBsAb) were monitored.From days 15 to 30 postinjection,the daily weight gain of the treated group was significantly increased(P<0.01) by 106.8%.A significant increase in the IGF-Ⅰ concentration and decrease in the SS level of the treated group were also observed.The results indicated that the combination of plasmid-mediated positive immunization against SS led to more robust weight gain in rabbits.

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