Position: Home > Articles > Effects of raw potato starch and organic acids on microbial community structure and tryptophan degradation in vitro
Animal Husbandry & Veterinary Medicine
2015,47
(11)
10-15
体外发酵体系中土豆淀粉和有机酸对猪肠道菌群结构及色氨酸代谢的影响
作 者：
尚伟伟;郑卫江;杜怀利;姚文
单 位：
南京农业大学动物科技学院
关键词：
接种粪样;土豆淀粉;有机酸;吲哚;粪臭素
摘 要：
旨在比较体外条件下,不同性别猪肠道菌群转化色氨酸产吲哚和粪臭素的能力,并研究土豆淀粉和有机酸的可能调控作用。试验分别以生长末(135 d)和育肥末(185 d)的母猪(F)、去势公猪(C)和公猪(M)粪便为接种物,进行两个批次的体外发酵试验。每种接种物均设5个处理:空白组(C)、色氨酸组(T)、土豆淀粉组(P)、有机酸组(O)和有机酸+土豆淀粉组(M);除C组外其余各组均含有0.1%色氨酸。结果表明:不同性别猪粪样发酵体系中氨氮水平存在显著差异(P<0.05),母猪高于公猪和去势猪;有机酸和土豆淀粉均能极显著降低氨氮水平(P<0.01)。土豆淀粉显著提高了TVFA、乙酸、丙酸和丁酸水平(P<0.05),并显著降低了支链脂肪酸的比例(P<0.05)。不同性别猪粪样发酵体系中吲哚和粪臭素水平差异显著(P<0.05),有机酸和土豆淀粉均能极显著降低吲哚和粪臭素水平(P<0.01)。菌群的DGGE图谱显示不同性别猪的菌群结构存在差异,土豆淀粉对菌群结构的调控作用较明显。由此可见,不同性别猪肠道菌群结构的差异可能导致了吲哚类物质产量的性别差异。有机酸和土豆淀粉均可有效降低吲哚类物质的产量,有机酸可能通过抑菌发挥作用,土豆淀粉可能通过改变菌群的结构和发酵类型实现。
译 名：
Effects of raw potato starch and organic acids on microbial community structure and tryptophan degradation in vitro
作 者：
SHANG Weiwei;ZHENG Weijiang;DU Huaili;YAO Wen;College of Animal Science and Technology,Nanjing Agricultural University;Key Laboratory of Animal Physiology and Biochemistry,Ministry of Agriculture;
关键词：
raw potato starch;;organic acids;;indole;;skatole;;in vitro fermentation
摘 要：
The present study was conducted to compare the capacity of fecal microbiata on converting tryptophan into indole and skatole and investigate the effect of raw potato starch and organic acids on microbial conversion of tryptophan. Two separate in vitro fermentation batches paralleled with pig age( 135 d and 185 d,respectively) were conducted by using feces from female( F),male( M) and castrated male( C)pigs as inocula. Each inoculum involved five treatments,blank medium only( C),medium with 0. 1% of tryptophan( T),medium with tryptophan and 1g of raw potato starch( P),medium with tryptophan and 1. 15 g of organic acids( O),and medium with tryptophan,organic acids and raw potato starch( M). The results indicated that remarkable differences in ammonia levels were observed among genders( P <0. 05),and organic acids and potato starch significantly decreased its levels( P<0. 01). Potato starch significantly increased the levels of TVFA,acetate,propionate and butyrate and clearly decreased the ratio of branched-chain fatty acid in TVFA( P< 0. 05). For indole and skatole levels,there were significant differences among genders( P< 0. 05),both organic acids and potato starch were capable of decreasing the levels of indole and skatole significantly( P<0. 01). DGGE patterns showed visible difference of microbial communities among genders and potato starch changed DGGE patterns clearly. Together,the structure difference of gut microbiota among pig genders might cause the gender-dependent production of indole and skatole. Both organic acids and potato starch can decrease their production effectively,while organic acids might play the role through inhibiting the growth of bacteria,potato starch might through modulate the structure of bacterial community.
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