当前位置: 首页 > 文章 > ~(60)Coγ辐射对不同品种草莓离体叶片再生及芽生长的影响 果树学报 2007,24 (3) 378-380
Position: Home > Articles > Effects of gamma radiation on shoot regeneration from leaf and bud growth of strawberry Journal of Fruit Science 2007,24 (3) 378-380

~(60)Coγ辐射对不同品种草莓离体叶片再生及芽生长的影响

作  者:
张慧琴;谢鸣;蒋桂华;陈昆松;吴庆;孙崇波;黄普乐;吴延军
单  位:
浙江省农业科学院作物与核技术研究所;浙江大学园艺系农业部园艺植物生长发育与生物技术重点开放实验室;浙江省农业科学院园艺研究所
关键词:
草莓;叶片;芽;60Coγ射线;增殖系数
摘  要:
以丰香和章姬草莓的无根无菌苗为试材,用10~40Gy 60Coγ射线辐照处理,以未辐照为对照,研究了60Coγ射线辐射对草莓叶片再生及植株继代增殖的影响。结果表明,叶片对辐射敏感,且丰香叶片比章姬叶片敏感,叶片再生的半致死剂量为10~20Gy;辐照后不同时间取叶接种,丰香叶片的不定芽再生率在剂量10Gy呈上升趋势,而其他剂量则几乎未见有变化,章姬在剂量10~30Gy,叶片的不定芽再生率提高较大,40Gy仍表现轻微下降;适宜的辐射剂量对草莓试管苗增殖有促进作用。
译  名:
Effects of gamma radiation on shoot regeneration from leaf and bud growth of strawberry
作  者:
ZHANG Hui-qin1,XIE Ming1,JIANG Gui-hua1,CHEN Kun-song2,WU Qing3,SUN Chong-bo1,HUANG Pu-le1,WU Yan-jun1 (1Zhejiang Horticultural Research Institute,Hangzhou,Zhejiang 310021 China; 2Department of Horticulture,Zhejiang University,The state Agriculture Ministry's Laboratory of Horticultural Plant Growth,Development and Biotechnology,Hangzhou,Zhejiang 310029 China; 3Zhejiang Research Institute of Crops and Atomic Technique Utilization,Hangzhou,Zhejiang 310021 China)
关键词:
Strawberry; Leaf; Bud; Radiation with gamma rays; Multiplication coefficient
摘  要:
The shoots of Akihime and Toyonoka strawberry cultivars in vitro were irradiated by gamma rays from 10 to 40 Gy and non-irradiation as control. The effects of gamma irradiation on shoot regeneration from leaf and multiplication coefficient of plantlets were studied. The results indicated that the leaf was very sensitive to the gamma irradiation and the leaf of Toyonoka was more sensitive than that of Akihime. The LD50 (50% decrease of generation) of leaf was genotype-dependent between 10 and 20 grays. The effect of different inoculation time after gamma rays irradiation on shoot regeneration from strawberry leaves in vitro,which increased at 10 Gy and almost no difference from 20 to 40 Gy for Toyonoka,which had very distinctly difference from 10 to 30 Gy and at the dose of 40 Gy descend slightly for Akihime. Suitable dose of gamma rays acted as a stimulus to the multiplication of strawberry in vitro.

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