Position: Home > Articles > Studies on Proliferation and Anti-oxidation Function of Rat Fast and Slow Muscle Satellite Cells by Energy Substances in Vitro
Progress in Veterinary Medicine
2015
(3)
76-83
能量物质在体外对大鼠快肌和慢肌卫星细胞增殖与抗氧化机能研究
作 者：
田苗苗;朱道立;王佳美;张网星;杨璐
单 位：
南通大学生命科学学院
关键词：
快肌卫星细胞;慢肌卫星细胞;过氧化氢;ATP;ADP;AMP
摘 要：
探讨体外能量物质对SD大鼠快肌(胫前肌)与慢肌(股中间肌)卫星细胞增殖功能和H2O2化学损伤修复研究。采用快、慢肌卫星细胞传代培养及分离纯化,四唑盐(MTT)比色法、DAPI荧光染色与免疫荧光抗体检测。结果表明:1与正常组比较,低剂量或中等剂量能量物质对快肌与慢肌卫星细胞有增殖机能,各剂量作用时间24h>48h>72h,其中以ATP溶液1.25mg/mL,ADP溶液1.25mg/mL,AMP溶液2.5mg/mL剂量促进快、慢肌卫星细胞增殖效果最显著;2 MTT比色法检测ATP、ADP及AMP修复损伤快、慢肌卫星细胞明显低于正常组卫星细胞而高于纯H2O2损伤卫星细胞。且修复效果表现为:ATP溶液以1.25mg/mL,ADP溶液为2.5mg/mL,AMP溶液为2.5mg/mL修复最佳;3模型组Bax抗体细胞数102.5±5.7,Bcl-2抗体细胞29.6±3.7;ATP、ADP及AMP溶液保护组Bax抗体细胞数67.3±4.9~72.8±5.4范围,而Bcl-2抗体细胞在69.8±5.1~78.5±4.9范围之内。慢肌股中间肌卫星细胞各组检测结果与其类似。
译 名：
Studies on Proliferation and Anti-oxidation Function of Rat Fast and Slow Muscle Satellite Cells by Energy Substances in Vitro
作 者：
TIAN Miao-miao;ZHU Dao-li;WANG Jia-mei;ZHANG Wang-xing;YANG Lu;School of Life Science,Nantong University;
关键词：
fast muscle satellite cells;;slow muscle satellite cells;;H2O2;;ATP;;ADP;;AMP
摘 要：
The repair study of H2O2 chemical injury and value-added function were investigated with energy substances for fast tibialis anterior muscle and slow vastus intermedius muscle satellite cells in vitro at SD rat.The satellite cells of fast and slow muscle were cultured by primary subculture and purification.The tetrazolium salt(MTT)assay,DAPI staining and immunofluorescence antibody were used for detection.The results indiciated:1 To compared with the normal group,low-dose or medium-dose ATP,ADP,and AMP were significant for satellite cell proliferation of fast and slow muscle in a variety of dosage time was24h>48h>72h.ATP solution 1.25mg/mL,ADP solution 1.25mg/mL,AMP solution 2.5 mg/mL dose facilitated fast and slow muscle satellite cell proliferation in the most significant effect.2 ATP,ADP,and AMP were found to repair fast muscle satellite cells damaged with tetrazolium detected by MTT assay,its activity was significantly lower than the normal group of satellite cells and was higher than pure H2O2 injury satellite cells.And the restore effect of performance:ATP solution 1.25mg/mL,ADP solution 2.5mg/mL,AMP solution of 2.5mg/mL were best restoration.3 Bax antibody cells in model group were 102.5±5.7,Bcl-2antibody cell number were 29.6±3.7;Bax antibody cells in ATP,ADP and AMP solution protective group were 67.3±4.9-72.8±5.4range,and Bcl-2antibody cells 69.8±5.1-78.5±4.9range.The results of slow muscle satellite cell groups tested were similar.
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