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当前位置: 首页 > 文章 > 基于液质联用技术的卵巢静止奶牛血浆代谢轮廓分析 中国兽医学报 2020 (5) 1047-1052
Position: Home > Articles > Plasma metabolic profiling of postpartum dairy cows with inactive ovaries based on LC/MS technique Chinese Journal of Veterinary Science 2020 (5) 1047-1052

基于液质联用技术的卵巢静止奶牛血浆代谢轮廓分析

作  者：
张锋;张江;白云龙;夏成;张洪友;徐闯
单  位：
关键词：
奶牛;卵巢静止;液相色谱/质谱联用技术;多元统计分析;差异代谢物
摘  要：
旨在应用代谢组学技术筛选产后卵巢静止奶牛血浆中差异代谢物,探究差异代谢物与奶牛卵巢静止的关系。试验选取了产后45～60 d卵巢静止奶牛和正常发情奶牛各15头,应用LC/MS技术检测两组奶牛血浆样品,运用多元统计分析与单变量分析,结合生物信息学分析,筛选出血浆差异代谢物,揭示不同代谢通路中的差异代谢物与奶牛卵巢静止的关系。结果显示:LC/MS分析共筛选出14种血浆差异代谢物。与发情组奶牛相比,卵巢静止组奶牛有11种差异代谢物明显升高:分别是D-塔格糖、L-亮氨酰-L-脯氨酸、L-鹅肌肽、脯氨酸-缬氨酸、赖氨酸-亮氨酸、N-(3-苯基丙酰基)甘氨酸、7-烯胆(甾)烷醇、γ-生育酚、β-隐黄素、视黄醛和鞘磷脂;3种差异代谢物降低:分别是胞嘧啶、1-棕榈酰溶血磷脂酰胆碱和1-硬脂酰-SN-甘油-3-磷酰胆碱。代谢通路分析代谢组学所获得的奶牛卵巢静止血浆差异代谢物,提示奶牛产后糖代谢、脂类代谢、氨基酸代谢和谷胱甘肽代谢等的异常会阻碍卵泡发育,从而导致奶牛产后发生卵巢静止。本试验获得了卵巢静止奶牛血浆差异代谢物,探究了奶牛产后卵巢静止发生中差异代谢物在糖代谢、脂代谢和氨基酸代谢以及其他代谢的变化,为今后深入研究奶牛卵巢静止的发病机理及防治新策略提供了方向。
译  名：
Plasma metabolic profiling of postpartum dairy cows with inactive ovaries based on LC/MS technique
作  者：
ZHANG Feng;ZHANG Jiang;BAI Yun-long;XIA Cheng;ZHANG Hong-you;XU Chuang;College of Animal Science and Veterinary Medicine,Heilongjiang Bayi Agricultural University;
关键词：
dairy cows;;inactive ovaries;;liquid chromatography/mass spectrometry;;multivariate statistical analysis;;differential metabolites
摘  要：
This trial aimed to screen plasma differential metabolites in postpartum dairy cows with inactive ovaries and estrous cows using metabolomics techniques to explore the relationship of plasma differential metabolites between two group cows.In this trial,15 inactive ovaries cows and 15 estrous cows(45-60 days postpartum) were selected,and LC/MS technique was used to analyze the plasma metabolic profiles of the cows,the multivariate statistical analysis and bioinformatics analysis were carried out to reveal the relationship between the differential metabolites in different metabolic pathways and the inactive ovaries of the cow.Fourteen plasma differential metabolites from two groups of cows were screened.Compared with the estrus cows,eleven different metabolites were significantly increased in the inactive ovaries cows,including D-tagatose,L-Leucyl-L-proline,L-goose carnosine,valine-valine,lysine-leucine,N-(3-phenylpropionyl) glycine,7-enebend Alkanol,γ-tocopherol,β-cryptoxanthin,retinal and sphingomyelin,respectively.Three differential metabolites were significantly decreased in the inactive ovaries cows,including cytosine,1-palmitoyl lysophosphatidylcholine and 1-stearoyl-SN-Glycerol-3-phosphorylcholine,respectively.The metabolic pathway analysis found that the plasma differential metabolites of dairy cow with inactive ovaries obtained by two metabolomics techniques were involved in glucose metabolism,lipid metabolism,amino acid metabolism and glutathione metabolism in dairy cows,indicating that those may hinder the development of follicles,leading to postpartum inactive ovaries in dairy cows.This study used LC/MS to screen the differential metabolites in plasma of inactive ovaries cows,and explores effect of differences metabolites on sugar metabolism,lipid metabolism and amino acid metabolism and other metabolism in the occurrence of postpartum inactive ovary cows.It provides a new direction for further research on the mechanism and control strategies of postpartum inactive ovary in dairy cows.

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