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当前位置: 首页 > 文章 > SMV与TRSV混合感染大豆叶片细胞的超微结构研究 大豆科学 1988 (1) 41-44+87-88+90
Position: Home > Articles > U1TRASTUCTURE OF MIXED PLANT VIRUS INFECTION SOYBEAN MOSAIC VIRUS WITH TOBACCO RINGSPOT VIRUS IN SOYBEAN Soybean Science 1988 (1) 41-44+87-88+90

SMV与TRSV混合感染大豆叶片细胞的超微结构研究

作  者：
李学湛
单  位：
黑龙江省农业科学院原子能利用研究所
关键词：
SMV;TRSV;混合感染;大豆叶片;超微结构
摘  要：
用两种RNA病毒(SMV、TRSV)混合感染大豆“Lee”叶片。通过对叶片细胞超微结构的连续观察,显示的主要病理特征是①SMV与TRSV粒体共栖于同一叶片细胞之中。②SMV和TRSV粒体量,分别多于同期单独感染寄主细胞的病毒粒体量,而TRSV粒体相对多于SMV粒体量。③经提纯SMV和TRSV产量高。负染后电镜所示:SMV粒体呈线状,长约750nm,直径约12nm、TRSV粒体呈多球形面体,直径约27nm。④病叶研磨液与已知SMV抗血清、TRSV抗血清作用均生成沉淀线,呈阳性反应。与BPMV抗血清作用呈阴性反应。
译  名：
U1TRASTUCTURE OF MIXED PLANT VIRUS INFECTION SOYBEAN MOSAIC VIRUS WITH TOBACCO RINGSPOT VIRUS IN SOYBEAN
作  者：
Li Xuezhan(Institute of Atomic Energy Utilization in Agriculture, Heilongjiang Academy of Agriculture Science. )
关键词：
SMV,TRSV,Mixed infection,Soybean leaf,Ultrastructure
摘  要：
Ultrastructural responses of soybean lee leaf cells simultaneously infected with two morphologically distinct RNA viruses soybean mosaic virus and tobacco ringspot virus were studied. The major characteristic of Soybean cells infected with two viruses included : (l)Tnere are two kind of viruses particles(SMV,TRSV)in the same cell of soybean lee. (2)The viruses particles are much more than single infection respectively. (3)The high yield were gotten after purification. The mixture of purified SMV and TRSV were negatively stained and examined in the electron microscope, Flexuous rods typical of SMV (750nm in length) and TRSV (27nm in diameter) icosahedra were observed, (4)SMV-TRSV in the Outchterlony double-diffusion test and SDS-immunodiffusion respectively produced precipitin band upon reaction with known antiserum of the Arkansas.

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