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Position: Home > Articles > Development and application of a combined technique of diagnosis for four kinds of parasitosis in cattle and sheep Animal Husbandry & Veterinary Medicine 2001,33 (3) 1-3

牛羊4种寄生虫病联合诊断技术的研究与应用

作  者:
孙仁寅;朱家新;张雪娟;刘恩勇;毛光琼;宋灿明;冯尚连;阳爱国
单  位:
安徽省安庆市畜牧兽医工作站;浙江省农业科学院畜牧?;四川省动物防疫监督总站;湖北省动物血防站;浙江省农业科学院畜牧兽医研究所;浙江省农业厅畜牧管理局
关键词:
寄生虫病;联合诊断技术;牛;羊
摘  要:
疫区牛羊同时寄生有捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫等多种寄生虫。目前血清学诊断方法对这 4种寄生虫病的检测需 2~ 4次操作。将捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫抗原分别以 0 1 5 ,0 0 4 ,0 0 6和 0 1 3μg/ μL的最佳浓度依次定点在同一硝酸纤维素膜条上 ,制成 4种寄生虫病联合诊断膜。用黄牛IgG ,水牛IgG和山羊IgG联合免疫兔 ,获得了高效价兔抗黄牛、水牛和山羊 (简称兔抗牛羊 )IgG抗血清。应用兔抗牛羊IgG抗血清连接酶标SPA ,建立了 1份血纸能同时用于检测牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病抗体技术。经浙江、湖北、四川、安徽等省应用 ,该项技术具有省工、省时、经济、实用等优点。适宜于牛、羊捻转血矛线虫、日本血吸虫、伊氏锥虫和肝片吸虫病流行区的普查或监测
译  名:
Development and application of a combined technique of diagnosis for four kinds of parasitosis in cattle and sheep
作  者:
SUN Ren yan 1,ZHU Jia xin 1,ZHANG Xue juan 2,LIU En yong 3, MAO Guang qiong 4,SONG Can ming 5,FENG Shang lian 2,YANG Ai guo 4 (1 Zhejiang Provincial Administration of Anim Husbandry,Hangzhou 310020,China; 2 Inst of Ani Husbandry and V
关键词:
parasitosis;combined diagnosis;cattle;sheep
摘  要:
Cattle and sheep could be simultaneously infected by Haemonchus contortus,Schisosoma japonicum,Trypanosoma evansi and Fasciola hepatica.Combined diagnosis kit was developed by adding four parasite antigen with the best work concentration 0 15,0 04,0 06 and 0 13 μg/μL on the same membrane of nitrocellulose.High titre antiserum was raised using IgG of cattle,buffalo and goat against rabbit.A multiple SPA Dot ELISA for Haemonchus contortus,Schisosoma japonicum,Trypanosoma evansi and Fasciola hepatica was established using antiserum linked SPA.

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