当前位置: 首页 > 文章 > 四季蜜龙眼花芽形成相关基因SAM的克隆 现代农业科技 2010 (23) 118-120
Position: Home > Articles > Cloning of SAM Gene Relate to Floral Bud Formation in Dimocarpus longan Lour. cv Sijimi Modern Agricultural Science and Technology 2010 (23) 118-120

四季蜜龙眼花芽形成相关基因SAM的克隆

作  者:
王晓航;陈清西;郑梦娇
单  位:
福建农林大学园艺学院
关键词:
四季蜜龙眼;成花相关基因;SAM基因
摘  要:
以四季蜜龙眼花芽为材料,进行了总RNA的提取与纯化,应用RT-PCR技术扩增其3′端序列,经测序和拼接,获得SAM3′末端,共1 253 bp;序列分析表明,与杨树SAM基因同源性达到96%,为龙眼成花机理的深入研究奠定了基础。
译  名:
Cloning of SAM Gene Relate to Floral Bud Formation in Dimocarpus longan Lour. cv Sijimi
作  者:
WANG Xiao-hang CHEN Qing-xi ZHENG Meng-jiao(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou Fujian 350002)
关键词:
Dimocarpus longan Lour.cv Sijimi;the relevant gene to floral bud formation;SAM gene
摘  要:
The extraction and purification of the RNA of flower bud of Dimocarpus longan Lour.cv Sijimi were conducted.Utilizing RT-PCR technique to expand 3' end sequence of SAM,testing its sequence and putting them together,the 3'end of SAM at the amount of 1 253 bp was attained.The nucleotide sequence comparison with the SAM gene of Populus trichocarpa showed that identity was higher than 96%,which would provide the basis to further study on the mechanism of blossom formation.

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