作 者：
买吾拉江·阿不都热衣木;李晨阳;李倩;徐芳;吉腾飞;赵军
单 位：
中国医学科学院北京协和医学院药物研究所天然药物活性成分与功能国家重点实验室;新疆大学生命科学与技术学院;新疆药物研究所维吾尔药重点实验室
摘 要：
[目的]采用高速逆流色谱法(HSCCC)分离纯化雪白睡莲花中的酚类成分。[方法]HSCCC分离过程分为两步,分别采用乙酸乙酯∶甲醇∶水(4∶1∶5)和乙酸乙酯∶正丁醇∶水(4∶1∶5)两个体系,固定相均采用上相,流动相均采用下相,流速为2.0 m L/min,主机转速设定为850 r/min,紫外检测波长为254 nm。高效液相色谱(HPLC)检测纯度。[结果]第一步从200 mg的睡莲花提取物中分离得到异槲皮苷(I,41.4 mg)、紫云英苷(II,40 mg)和一混合物85 mg;第二步从混合物中分离纯化得到isostrictiniin(III,50.1 mg)和烟花苷(IV,26.3 mg),上述4种物质的纯度分别为73.53%、92.42%、82.36%和85.65%。[结论]HSCCC可快速的分离纯化雪白睡莲花中的酚类成分。
译 名：
Isolation and Purification of Phenolic Compounds from Nymphaea candida Presl by High-speed Counter-current Chromatography
作 者：
ABUDUREYIMU Maiwylajiang;LI Chen-yang;LI Qian;ZHAO Jun;College of Life Sciences & Technology,Xinjian University;Xinjiang Key Laboratory for Uighur Medicines,Xinjiang Institute of Materia Medica;
关键词：
High-speed counter-current chromatography;;Nymphaea candida;;Phenolic compounds
摘 要：
[Objective] To isolate and purify phenolic constituents from Nymphaea candida Presl by high-speed counter-current chromatography( HSCCC). [Methods] HSCCC was employed to separate phenolic compounds from N. candida with two steps. Two solvent systems were ethyl acetate ∶ methanol ∶ water( 4 ∶ 1 ∶ 5) and ethyl acetate ∶ n-butanol ∶ water( 4 ∶ 1 ∶ 5). HSCCC was executed under the conditions of2. 0 m L / min flow rate,850 r / min rotation speed and 254 nm detection wavelength. The purities of compounds were determined by HPLC.[Result]In the first separation step,isoquercitrin( I,41. 4 mg),astragalin( II,40 mg) and mixtures of III and IV( 85 mg) were extracted from 200 mg sample. In the second step,mixtures of III and IV were obtained: isostrictiniin( III,50. 1 mg) and nicotiflorin( IV,26. 3 mg).The purities of these compounds were determined as 73. 53%,92. 42%,82. 36% and 85. 65% by HPLC. [Conclusion]Phenolic compounds from N. candida can be quickly isolated and purified by HSCCC.
