中国农业期刊集成服务平台

当前位置: 首页 > 文章 > Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen 农业科学学报 (英文) 2014,13 (4)

Position: Home > Articles > Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen Journal of Integrative Agriculture 2014,13 (4)

Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen

作者：

Wang Xiao-li;Yu Wen-juan;Zhou Qian;Han Rui-feng;Huang Dan-feng

单位：

Shanghai Jiao Tong Univ, Sch Agr & Biol, Dept Hort, Shanghai 200240, Peoples R China;Shanghai Jiao Tong Univ, Instrumental Anal Ctr, Shanghai 200240, Peoples R China

关键词：

amino acid;metabolite profile;pakchoi

摘要：

Different nitrogen (N) forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this study was to identify primary metabolites associated with amino acid-N (Gly, Gin and Ala) through metabolic profile analysis using gas chromatography-mass spectrometry (GC-MS). Plants of pakchoi (Brassica campestris L. ssp. chinensis L.), Huawang and Wuyueman cultivars, were grown with different nitrogen forms (i.e., Gly, Gln, Ala, NO3--N, and N starvation) applied under sterile hydroponic conditions. The fresh weight and plant N accumulation of Huawang were greater than those of Wuyueman, which indicates that the former exhibited better N-use efficiency than the latter. The physiological performances of the applied N forms were generally in the order of NO3--N>Gln>Gly>Ala. The metabolic analysis of leaf polar extracts revealed 30 amino acid N-responsive metabolites in the two pakchoi cultivars, mainly consisting of sugars, amino acids, and organic acids. Changes in the carbon metabolism of pakchoi leaves under amino acid treatments occurred via the accumulation of fructose, glucose, xylose, and arabinose. Disruption of amino acid metabolism resulted in accumulation of endogenous Gly in Gly treatment, Pro in Ala treatment, and Asn in three amino acid (Gly, Gin and Ala) treatments. By contrast, the levels of endogenous Gin and Leu decreased. However, this reduction varied among cultivars and amino acid types. Amino acid-N supply also affected the citric acid cycle, namely, the second stage of respiration, where leaves in Gly, Gin and Ala treatments contained low levels of malic, citric and succinic acids compared with leaves in NO3--N treatments. No significant difference in the metabolic responses was observed between the two cultivars which differed in their capability to use N. The response of primary metabolites in pakchoi leaves to amino acid-N supply may serve an important function in pakchoi adaptation to amino acid-N sources.

相似文章

Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs [Chen Liang, Gao Li-xiang, Liu Li, Ding Ze-ming, Zhang Hong-fu] 农业科学学报 (英文) 2015,14 (12)
High Temperature at Grain-filling Stage Affects Nitrogen Metabolism Enzyme Activities in Grains and Grain Nutritional Quality in Rice [Chen Liang, Liping Chen, Yan Wang, Jia Liu, Guangbiao Xu, Li Tia] 水稻科学(英文版) 2011,18 (3)
Application Effects of Amino Acid Powder, Fermented Rapeseed Meal and Fermented Cottonseed Meal in Feeds for AIIogynogenetic Crucian Carp [Kong, Qing Li, Bo Zhang, Hsi Hsien Wei, Jianhuai Ye, Yuan-tu, DAIXiu-ying, Hong Wang, Wenjuan, Cai Dan, Chunfan] 动物与饲料科学(英文版) 2010,2 (6)
Interactive effects of elevated carbon dioxide and nitrogen availability on fruit quality of cucumber (Cucumis sativus L.) [Dong Jin-long, Li Xun, Gruda, Nazim, Duan Zeng-qiang] 农业科学学报 (英文) 2018,17 (11)
Effects of the severity and timing of basal leaf removal on the amino acids profiles of Sauvignon Blanc grapes and wines [Yue Xiao-feng, Ju Yan-lun, Tang Zi-zhu, Zhao Ya-meng, Jiao Xu-liang, Zhang Zhen-wen] 农业科学学报 (英文) 2019,18 (9)
Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen [Xiaoli Wang, Wei Yu, Qian Zhou, Ruifeng Han, Danfeng Huan] 农业科学学报(英文版) 2014,13 (4)
Effect of graded levels of fiber from alfalfa meal on apparent and standardized ileal digestibility of amino acids of growing pigs [Liang Chen, Le Gao, Li Liu, Ze-ming Ding, Hongfu Zhan] 农业科学学报(英文版) 2015,14 (12)
Characterization of Amino Acid Composition in Fruits of Three Rosa roxburghii Genotypes [Min Lü, Hongyu An, Daoping Wan] 园艺学报(英文版) 2017,3 (6)
Optimization of High-Protein Glutinous Rice Flour Production Using Response Surface Method [Kanjanapa Eakkanaluksamee, Jirarat Anuntagoo] 水稻科学(英文版) 2020,27 (1)
高效液相色谱技术在饲料安全生产中的应用 [Li Xi] 畜牧与饲料科学 2009,30 (1) 99-101

导出引用

计量

文章访问数: 14

HTML全文浏览量: 0

PDF下载量: 0

Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen

相似文章

所属期刊

农业科学学报 (英文)

推荐期刊

农业科学学报 (英文)