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Position: Home > Articles > Determination of Fluoroquinolone Residues in Pork by HPLC MEAT RESEARCH 2011 (10) 29-31

高效液相色谱法测定猪肉中氟喹诺酮类药物

作  者:
朱莉萍;朱涛;武娜;倪永付;王勇;闫秋成
单  位:
济宁出入境检验检疫局
关键词:
高效液相色谱法;氟喹诺酮类药物;残留分析;猪肉
摘  要:
采用高效液相色谱-荧光法(HPLC-FLD)检测猪肉中两种氟喹诺酮类药物。猪肉样品经无水硫酸钠脱水后用1%醋酸+乙腈提取,提取液用正己烷饱和的乙腈净化,用流动相(乙腈、0.01mol/L磷酸二氢钠(磷酸调pH3)体积比10:90)定容。通过对猪肉样品中氟喹诺酮类药物的检测表明该方法具有准确、快速、灵敏的特点。
译  名:
Determination of Fluoroquinolone Residues in Pork by HPLC
作  者:
ZHU Li-ping,ZHU Tao,WU Na,NI Yong-fu,WANG Yong,YAN Qiu-cheng(Jining Import and Export Inspetion and Quaratine Bureau,Jining 272000,China)
关键词:
high performance liquid chromatography(HPLC);fluoroquinolone;residue analysis;pork
摘  要:
An HPLC with FLD(fluorescence detector) method was established for the determination of two kinds of fluoroquinolones in pork.Pork samples were extracted with 1% acetic acid/acetonitrile,followed by water removal by anhydrous sodium sulfate and clean-up by acetonitrile saturated n-hexane.The mobile phase was composed of acetonitrile and 0.01 mol/L sodium phosphate(pH 3,adjusted with phosphorous acid) at a ratio of 10:90(V/V).The analytes were separated on a ZORBAX-SB-C18 column and detected by a fluorescence detector.The established method proved accurate,rapidy and sensitive.

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