摘 要：
将100只新西兰白兔随机分为5组,即对照组,模型组及中药提取物高、中、低剂量组,除对照组外,试验兔感染1×105个肠艾美耳球虫卵囊,再分别每天灌服0、0.500、0.250、0.125 g/kg的中药提取药物(白头翁、柴胡、半夏、黄芩、槟榔、甘草等组成),每天灌服1次,连续使用3 d,给药后连续观察21 d,每天记录试验兔的死亡和血便情况,隔7d测量试验兔体质量,采集粪便计数粪便卵囊数(OPG);试验结束后剖杀动物,观察其盲肠病变情况。结果显示:经中药提取物高剂量和中剂量治疗的试验兔存活率分别达90%和85%,感染球虫的程度逐渐由严重转为轻度,模型组和低剂量组试验兔的存活率分别为25%和50%,且存活试验兔大部分感染严重;在给药后7、14、21 d,高剂量组和中剂量组试验兔的体质量显著高于模型组的(P<0.05),OPG极显著低于模型组的(P<0.01),血便记分显著低于模型组的(P<0.05);给药后7、14 d,低剂量组试验兔的OPG极显著低于模型组的(P<0.01);给药后14 d,低剂量组试验兔的体质量显著高于模型组的(P<0.05);给药后21 d,高剂量组和中剂量组试验兔的增质量极显著高于模型组的(P<0.01),显著高于低剂量组的(P<0.05),相对增质量极显著高于模型组和低剂量组的(P<0.01),OPG极显著低于低剂量组的(P<0.01),血便消失。高、中、低剂量的中药提取物组试验兔的抗球虫指数极显著高于模型组的(P<0.01),治疗效果分别为高效、中效、无效。可见,在本研究剂量范围内,治疗效果存在一定的量效关系,高剂量治疗效果最佳。
译 名：
Resistance effects of traditional Chinese medicine compound extract on the rabbit coccidian
作 者：
LUO Yichen;ZHU Maixun;LIN Chunfa;LIU Zirui;CAO Maoyu;College of Animal Science, Southwest University;Chongqing Academy of Animal Sciences;
关键词：
rabbit;;traditional Chinese medicine compound extract;;coccidial oocyst;;treatment effect
摘 要：
In the study, 100 New Zealand white rabbits were randomly grouped into 5 groups, control group, model group, Chinese herb extracts treated high, medium and low dose group. Except the control group, each group of experimental rabbits infected 1×105 eggs of Eimeria intestinalis were treated with 0, 0.500, 0.250, 0.125 g/kg traditional Chinese medicine compound extract(composed of PULSATILLAE RADIX, BUPLEURI RADIX, PINELLIAE RHIZOMA, SCUTELLARIAE RADIX, ARECAE SEMEN and GLYCYRRHIZAE RADIX ET RHIZOMA) for 3 d with once per day, and 21 d continuous observation after the treatment. The observation included the every day deaths and bloody stool, the every 7 d body mass and collected dung as OPG, the caecal lesions of rabbits by dissection at the end of the test. The results showed that the survival rate of experimental rabbits treated with high-dose and medium-dose traditional Chinese medicine compound extract reached 90% and 85%, and the degree of coccidiosis infection gradually changed from severe to mild. While, the model group and low-dose group was at 25% and 50%, and most of the survival experimental rabbits were seriously infected. At 7, 14, and 21 d after the administration, the body weight of the experimental rabbits in the high-dose and middle-dose groups were significantly higher than the model group(P<0.05), the OPG was significantly lower than the model group(P<0.01), and the blood stool score was significantly lower than model group(P<0.05). At the 7 d administration, only the OPG of the experimental rabbits in low-dose group was significantly lower than the model group(P<0.01). At the 14 d administration, the body weight of the experimental rabbits in low-dose group was significantly higher than the model group(P<0.05), the OPG was significantly lower than the model group(P<0.01). At the 21 d administration, the body weight of the experimental rabbits in the high-dose and middle-dose groups were significantly higher than the model group(P<0.01) and significantly lower than the low-dose group(P<0.01), relative mass gain was significantly higher than the model group and low-dose group(P<0.01), the OPG was significantly lower than the model group and low-dose group(P<0.01), and blood disappeared. In addition, the ACI value of the high, middle and low-dose group were significantly higher than that of the model group(P<0.01), and the treatment effects were high, medium, and ineffective. These results indicated that the traditional Chinese medicine compound extract had a good therapeutic effect on coccidian, and the higher dose treatment has the better effect.
