中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 金钱鱼cyp17a1基因克隆、组织分布及在卵巢发育中的表达 广东海洋大学学报 2018 (5) 1-7
Position: Home > Articles > Cloning,Tissue Distribution and Expression of cyp17a1 at Different Ovarian Development Stages in Spotted Scat(Scatophagus argus) Journal of Guangdong Ocean University 2018 (5) 1-7

金钱鱼cyp17a1基因克隆、组织分布及在卵巢发育中的表达

作  者：
翟毅;刘建业;贾丽英;江东能;陈华谱;李广丽;邓思平
关键词：
金钱鱼;cyp17a1;基因克隆;组织分布;基因表达;卵巢发育
摘  要：
【目的】克隆金钱鱼cyp17a1基因,分析其在组织中的分布、卵巢不同发育时期中的表达变化,为金钱鱼繁殖生物学提供理论依据。【方法】通过金钱鱼转录组文库筛选和RT-PCR进行cyp17a1 c DNA序列扩增;用DNAstar软件比较Cyp17a1同源性,MEGA5.0构建系统进化树,RT-PCR检测cyp17a1组织表达,实时定量PCR检测不同卵巢发育时期cyp17a1的表达量。【结果】金钱鱼cyp17a1开放阅读框编码515个氨基酸残基;Cyp17a1氨基酸序列与同属鲈形目鱼类Cyp17a1同源性较高(87.8%～92.7%),与大黄鱼最高(92.7%),与其他目鱼类同源性次之(72.7%～85.0%),与硬骨鱼类Cyp17a2或Cyp17a1-like同源性最低(48.2%～51.7%);所有硬骨鱼类Cyp17a1聚为一支,在Cyp17a1分支中,金钱鱼与鲈形目鱼类聚为一支;cyp17a1在性腺中表达量较高,在精巢中表达最强;在肝、脑和头肾表达较弱;在脾脏、肠、心脏、肌肉和鳃中未检测到表达;卵巢中cyp17a1的表达量随卵巢发育逐渐增加,Ⅳ期卵巢中cyp17a1表达量最高。【结论】cyp17a1在性腺中表达量较高,在金钱鱼卵巢发育过程中有重要作用。
译  名：
Cloning,Tissue Distribution and Expression of cyp17a1 at Different Ovarian Development Stages in Spotted Scat(Scatophagus argus)
作  者：
ZHAI Yi;LIU Jian-ye;JIA Li-ying;JIANG Dong-neng;CHEN Hua-pu;LI Guang-li;DENG Si-ping;Fisheries College, Guangdong Ocean University;Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species;Marine Ecology and Aquaculture Environment of Zhanjiang;
单  位：
ZHAI Yi%LIU Jian-ye%JIA Li-ying%JIANG Dong-neng%CHEN Hua-pu%LI Guang-li%DENG Si-ping%Fisheries College, Guangdong Ocean University%Guangdong Research Center on Reproductive Control and Breeding Technology of Indigenous Valuable Fish Species%Marine Ecology and Aquaculture Environment of Zhanjiang
关键词：
Scatophagus argus;;cyp17a1;;gene clone;;tissue distribution;;gene expression;;ovarian development stages
摘  要：
【Objective】To provide a theoretical basis for the reproductive biology of spotted scat(Scatophagus argus), the cyp17 a1 gene was cloned and analyzed. The tissue distribution and expression of the hypothalamus at different ovarian development stages were also evaluated. 【Methods】The cyp17 a1 cDNA was amplified by the combination of transcriptome and RT-PCR. The alignments of the amino acid sequences of Cyp17 a1 were performed using DNAstar. A phylogenetic tree was constructed by using MEGA 5.0. Tissue distribution of Cyp17 a1 expression was analyzed by RT-PCR. The expression of cyp17 a1 at ovarian developmental stages was detected by real-time quantitative PCR.【Results】The cyp17 a1 open reading frame encodes a predicted protein of 515 amino acid(aa) residues. The Cyp17 a1 is highly homologous with that of other Cyp17 a1(72.7%–92.7%), and has low homology with that of Cyp17 a2 or Cyp17 a1-like(48.2%–51.7%) in teleost. The Cyp17 a1 and other teleost Cyp17 a1 were clustered into one clade. The spotted scat clustered firmly with other Perciformes fish. RT-PCR showed that the expression of the cyp17 a1 was higher in the gonads, with the strongest expression in the testis, but weak in the liver, brain and no expression in head kidney, spleen, intestines, heart, muscle and gills. The cyp17 a1 expression increased gradually with ovarian development, and the cyp17 a1 transcript level in phase III was significantly higher than that in phase II, and the cyp17 a1 in phase IV was significantly higher than that in phase III.【Conclusion】The cyp17 a1 has a high expression level in gonads. It plays an important role during the ovarian development in spotted scat.

相似文章

导出引用
计量
文章访问数: 12
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46