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当前位置: 首页 > 文章 > 赤水乌骨鸡TYR基因多态性及生物信息学分析 南方农业学报 2019 (12) 2806-2811
Position: Home > Articles > Polymorphism and bioinformatics analysis of TYR gene in Chishui Black-bone Chicken Journal of Southern Agriculture 2019 (12) 2806-2811

赤水乌骨鸡TYR基因多态性及生物信息学分析

作  者：
祖盘玉;李维;林家栋;李洪林;刘洋;牟腾慧;龙广丽;简华峰;张福平
单  位：
贵州大学动物科学学院/高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室;贵州省畜禽遗传资源管理站
关键词：
赤水乌骨鸡;泰和乌鸡;TYR基因;SNP位点;生物信息学
摘  要：
【目的】找出赤水乌骨鸡和泰和乌鸡TYR基因外显子的多态位点(SNP),为后期利用分子遗传学技术选育乌度更深的赤水乌骨鸡提供参考依据。【方法】采用DNA混池结合PCR产物直接测序法,分析赤水乌骨鸡和泰和乌鸡TYR基因外显子的SNP位点,并对筛选出的SNP位点进行生物信息学分析。【结果】在泰和乌鸡中发现4个SNPs位点,分别为Exon1-C829T、Exon1-C921T、Intron1-A1018G和Intron2-T79A,其中,Exon1-C829T和Exon1-C921T为同义突变;在赤水乌骨鸡中发现5个SNPs位点,分别为Intron1-G156A、Intron2-T7C、Intron4-G201A、Intron4-C221T和Exon5-A205G(非编码区)。利用在线生物信息学分析软件对TYR基因突变前后编码区序列的mRNA二级结构进行预测分析,结果发现赤水乌骨鸡的mRNA二级结构未发生变化,而泰和乌鸡Exon1-C829T和Exon1-C921T的突变均引起mRNA二级结构改变,使得TYR基因m RNA二级结构最小自由能减小,即二级结构稳定性增加。赤水乌骨鸡TYR蛋白二级结构由α-螺旋、无规则卷曲、β-转角及扩展链组成,分别占26.65%、55.31%、3.41%和14.63%。【结论】Exon1-C921T突变可能会对鸡的肉色产生影响,故推测Exon1-C921T是影响赤水乌骨鸡和泰和乌鸡乌度差异的原因之一。
译  名：
Polymorphism and bioinformatics analysis of TYR gene in Chishui Black-bone Chicken
作  者：
ZU Pan-yu;LI Wei;LIN Jia-dong;LI Hong-lin;LIU Yang;MOU Teng-hui;LONG Guang-li;JIAN Hua-feng;ZHANG Fu-ping;College of Animal Sciences,Guizhou University/Key Laboratory of Animal Genetics,Breeding and Reproduction in the Plateau Mountainous Region,Ministry of Education/Guizhou Key Laboratory of Animal Genetics,Breeding and Reproduction;Guizhou Management Station of Livestock Genetic Resources;
关键词：
Chishui Black-bone Chicken;;Taihe Silky Fowl;;TYR gene;;SNPs;;bioinformatics
摘  要：
【Objective】To find the polymorphic loci(SNP)of exon of TYR gene from Chishui Black-bone Chicken and Taihe Silky Fowl,and lay a foundation for the later cultivation of Chishui Black-bone Chicken with deeper Wudu by molecular genetics technology.【Method】The TYR gene exon SNPs of Chishui black-bone chicken and Taihe Silky Fowl were analyzed by DNA pooling and direct sequencing of PCR products,and bioinformatics analysis on SNP selected was conducted.【Result】It was found that there were four SNPs in Taihe Silky Fowl,namely:Exon1-C829 T,Exon1-C921 T,Intron1-A1018 G,Intron2-T79 A,among which Exon1-C829 T and Exon1-C921 T were synonymous mutations. Five SNPs were found in Chishui Black-bone Chicken,they were Intron1-G156 A,Intron2-T7 C,Intron4-G201 A,Intron4-C221 T,Exon5-A205 G(non-coding region). The bioinformatics analysis software was used to analyze the mRNA secondary structure of coding region of TYR gene before and after mutation. It was found that mRNA secondary structure of Chishui Black-bone Chicken did not change,but both Taihe Silky Fowl Exon1-C829 T and Exon1-C921 T caused changes in the secondary structure of m RNA,which resulted in the minimum free energy of the secondary structure of TYR gene mRNA,that was,the stability of the secondary structure increases. TYR protein secondary structure of Chishui Blackbone Chicken was consisted of α-helix,random coil,β-turn and extended chain which accounted for 26.65%,55.31%,3.41% and 14.63%.【Conclusion】Exon1-C921 T mutation may change the color of chicken meat. Exon1-C921 T may be one of the reasons that affect the difference between Chishui Black-bone Chicken and Taihe Silky Fowl.

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