Study on the Adventitious Roots Tissue Culture of Valeriana fauriei var. dasycarpa Hara

LI Mei-yang;JIN Jiang-shan;College of Agriculture, Yanbian University;

Valeriana fauriei var.dasycarpa Hara;;bioreactor;;valerenic acid;;valepotriate;;sucrose concentration;;nitrogen sources;;phosphate sources

To explore the effects of different culture conditions on Valeriana fauriei var. dasycarpa Hara adventitious roots growth and its effective components synthesis,the adventitious roots were obtained through tissue culture by manipulation of sucrose concentration, nitrogen and phosphate sources and the growth of plant, the content of valepotriates and valerenic acids were determined by HPLC. The results showed that when the sucrose concentration of 60 g / L were favorable for growth of adventitious roots and total valepotriates and valerenic acids production. The maximum root growth rates and content of valepotriates and valerenic acids were achieved while NO3-/ NH4+was 1 ∶1. To compare the media with adding Na H2PO4 and the medium without adding Na H2PO4,changing of Na H2PO4 concentration could favor for the adventitious root growth. But high Na H2PO4 concentration inhibited valepotriates and valerian acid biosynthesis. When Na H2PO4 in the basic culture medium of0.3 times, growth of adventitious roots reached a maximum production of 14.89 g / L,growth rate was the highest, reached16.18 times, total valerenic acids content was up to the highest yield 13.88 mg / L.