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当前位置: 首页 > 文章 > 一株根际好氧反硝化菌的筛选及其反硝化条件研究 土壤 2013,45 (4) 683-690
Position: Home > Articles > Isolation and Denitrification Conditions of Rhizosphere Aerobic Denitrifying Bacterium RWX31 Soils 2013,45 (4) 683-690

一株根际好氧反硝化菌的筛选及其反硝化条件研究

作  者：
周影茹;陆玉芳;施卫明
单  位：
土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所)
关键词：
根际;好氧反硝化菌;硝态氮去除率;条件研究
摘  要：
为丰富好氧反硝化菌株种类,本文从不同环境样品中富集筛选好氧反硝化细菌,最终得到一株高效根际菌株RWX31,其在初始3NO-N浓度140 mg/L时24 h去除率为82%,并在好氧条件下可进行反硝化作用产生N2O。通过单因素试验研究该菌株进行反硝化作用的条件和特性,结果表明,菌株RWX31最适培养基条件分别为:以柠檬酸钠为碳源,接种量为1%,Mg2+浓度为0.05 g/L,反硝化初始氮源中2NO-N比例为0。最适培养条件为:温度28℃~32℃,pH为7.0~7.5,C/N为8~12,DO浓度约6.5~7.0 mg/L。在这些培养条件下菌株3NO-N去除率可增至90%以上。菌株RWX31的3NO-N去除能力高于以往报道的反硝化菌株,是一株具有实际应用价值和潜力的菌株。
译  名：
Isolation and Denitrification Conditions of Rhizosphere Aerobic Denitrifying Bacterium RWX31
作  者：
ZHOU Ying-ru;LU Yu-fang;SHI Wei-ming;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science,Chinese Academy of Sciences);University of Chinese Academy of Sciences;
关键词：
Rhizosphere,Aerobic denitrifying,Nitrate nitrogen removal efficiency,Condition investigation
摘  要：
The aim of this study was to screen aerobic denitrifying strain from various environmental sites and to investigate the denitrification conditions for the isolated strain. Enrichment culture technique was used in screening and acclimatization of strain, a rhizosphere aerobic denitrifying bacterium RWX31 was isolated and single-factor experiments were used to investigate the denitrification conditions and characteristics. The nitrate remove efficiency of aerobic denitrifying strain RWX31 was 82% when cultivated in 140 mg/L nitrate for 24 hours, N2O could be produced aerobically through aerobic denitrification of strain RWX31. The optimum medium was: sodium citrate as carbon source, 1%inoculation volume, 0.05 g/L Mg2+concentration and nitrite proportion of 0 in the initial denitrification nitrogen source.The optimum condition was: temperature 28℃-32℃, pH 7.0-7.5, C/N ratio 8-12 and DO concentration 6.5-7.0 mg/L.The highest nitrate removal efficiency was higher than 90% under the above conditions. Rhizosphere bacterium RWX31was an efficient aerobic denitrifying strain with higher nitrate removal efficiency than reported previously and has application potential.

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