当前位置: 首页 > 文章 > 药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用 浙江农业学报 2014,26 (3) 680-684
Position: Home > Articles > Study of medicinal plant Epimedium brevicornum Maxim and platelet effects on chondrocyte proliferation Acta Agriculturae Zhejiangensis 2014,26 (3) 680-684

药用植物淫羊藿与富血小板血浆协同对软骨细胞的增殖作用

作  者:
张蕾;程瑜;金王东;王飘逸;单乐天
单  位:
浙江科技学院;浙江中医药大学
关键词:
骨性关节炎;淫羊藿;富血小板血浆;软骨细胞;细胞增值
摘  要:
观察淫羊藿和富血小板血浆(PRP)对SD大鼠软骨细胞增殖作用的影响,探讨两者对软骨细胞的协同作用。取3周龄SD大鼠的膝关节软骨建立软骨细胞体外培养体系,分别采用MTT法、流式细胞术对细胞增殖作用进行检测。结果表明:(1)PRP对软骨细胞增殖有显著的促进作用(P<0.01);(2)淫羊藿对软骨细胞有一定的增殖作用,但不显著;(3)淫羊藿联合PRP对软骨细胞的增殖作用显著下降,效果低于淫羊藿或PRP组(P<0.01)。淫羊藿联合PRP对软骨细胞有显著抑制作用(P<0.01),推断淫羊藿某些成分抑制了PRP中生长因子的活性,从而减弱了PRP对软骨细胞的作用,该推断有待进一步研究确认。
译  名:
Study of medicinal plant Epimedium brevicornum Maxim and platelet effects on chondrocyte proliferation
作  者:
ZHANG Lei;CHENG Yu;JIN Wang-dong;WANG Piao-yi;SHAN Le-tian;Zhejiang University of Science & Technology;Zhejiang Provincial Key Lab for Chem-&Bio-Processing Technology of Agricultural Products;Zhejiang Chinese Medical University;
关键词:
osteoarthritis;;Epimedium brevicornum Maxim;;platelet-rich plasma;;chondrocytes;;proliferation
摘  要:
To observe the proliferative effects of Epimedium brevicornum Maxim and platelet-rich plasma( PRP),respectively,on SD rat chondrocyte,and to explore their synergic effect between each other,in vitro chondrocyte culter system was established using 3-week-old SD rat knee cartilage. MTT and flow cytometry assays were adopted to test the chondrocyte prolifereation. Results:( 1) PRP has significant proliferative effect on chondrocyte( P < 0. 01);( 2) Epimedium brevicornum Maxim has a bit proliferative effect on chondrocyte;( 3) Epimedium brevicornum Maxim combined with PRP has inhibitory effect on chondrocyte( P < 0. 01). Epimedium brevicornum Maxim combined PRP has inhibitory effect on chondrocyte( P < 0. 01),deducing that some components in Epimedium brevicornum Maxim inhibited PRP growth factors and then decrease PRP activity. The conclusion needs further investigation.

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