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Position: Home > Articles > Digoxin - Labelled P - type Pili Gene Probe for the Detection of Escherichia coli Strains from Chickens China Animal Health Inspection 2000 (10)

地高辛标记P菌毛基因检测探针鸡大肠杆菌

作  者:
戴鼎震;龚大春;杨待建;张冠卿
单  位:
南京农业大学;湖北农学院
关键词:
P型菌毛;核酸探针;鸡大肠杆菌;检测
摘  要:
用地高辛标记鸡大肠杆菌P型菌毛结构基因(PapA)制成核酸探针,作斑点杂交试验,检测28个血清型鸡大肠杆菌50个菌株与该探针的同源性。结果表明有5个菌株被检测为阳性,阳性率为10%。
译  名:
Digoxin - Labelled P - type Pili Gene Probe for the Detection of Escherichia coli Strains from Chickens
作  者:
Dai Dingzhen; Gong Dachun; Yang Daijian (Nanjing Agricultaral University, Nanjing 210095)
关键词:
Type P Pile Nucleic Acid Probe Escherichia coli.
摘  要:
A nucleic acid probe was prepared by using Type P pill gene labelled with digoxin. 50 strains of 28 serotypes of Escherihia colt were detected for the homogenicity with the probe by dot - blot hybridization test. It was found that 5 out of 50 tested E. colt strains were homogenetic with the probe. The positive rate was 10%.

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