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Position: Home > Articles > Detection of Seed Purity of Two-line Hybird Rice “Quanliangyou 2118” by SSR Marker Technique Journal of Anhui Agricultural Sciences 2020 (1) 42-43

SSR分子标记鉴定两系杂交水稻“荃两优2118”的种子纯度

作  者:
王立广;张翔;黄贯刘;汪仁举;康圣好;熊延军;张奎
单  位:
安徽华安种业有限责任公司
关键词:
SSR分子标记;两系杂交水稻;种子纯度;田间鉴定
摘  要:
采用SSR分子标记技术鉴定两系杂交水稻"荃两优2118"的种子纯度。从48对SSR引物中筛选到2组引物(RM7120、RM8277)。随机选取"荃两优2118"种子6组,每组样品随机取192棵种子苗种进行纯度检测,结果显示同一样品SSR标记与田间鉴定结果接近,最大、最小差异分别为2.05%和0.23%。因此,SSR标记技术适用于"荃两优2118"的种子纯度鉴定。
译  名:
Detection of Seed Purity of Two-line Hybird Rice “Quanliangyou 2118” by SSR Marker Technique
作  者:
WANG Li-guang;ZHANG Xiang;HUANG Guan-liu;Anhui Hua'an Seed Co., Ltd.;
关键词:
Simple sequence repeat(SSR) molecular markers;;Two-line hybrid rice;;Seed purity;;Field identification
摘  要:
The seed purity of two-line hybird rice "Quan liangyou 2118" was identified using simple sequence repeat(SSR) molecular markers. Two pairs of primers(RM7120 and RM8277) were screened out from 48 pairs of SSR primers. A total of 2 pairs of primers were used to test the purity of 6 groups of the "Quan liangyou 2118", with 192 seed seedings in each group. The SSR identification results were basically agreed with the field identification results, the difference of which ranged from 0.23%-2.05%. The research results indicated that SSR markers could be utilized to identify the purity of "Quan liangyou 2118".

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