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Position: Home > Articles > Cloning and sequence analysis of two cecropin D genes from the common cutworm,Spodoptera litura (Lepidoptera: Noctuidae) Acta Entomologica Sinica 2007,50 (7) 100-104

斜纹夜蛾两个天蚕素D基因的克隆及序列分析

作  者：
陈维春;宋杰;庞义
单  位：
中山大学有害生物控制与资源利用国家重点实验室;广东医学院寄生虫学教研室
关键词：
斜纹夜蛾;抗菌肽;天蚕素;内含子;基因结构
摘  要：
天蚕素是昆虫抵御病菌入侵的一类抗菌肽家族。根据斜纹夜蛾Spodoptera litura天蚕素B基因设计特异引物,通过PCR扩增得到2个新的天蚕素基因部分序列,分别命名为cecD1和cecD2(GenBank登录号分别为EF555567和EF555568)。2个基因编码同一个天蚕素D蛋白,该蛋白的成熟肽与天蚕素B存在2个氨基酸残基差异。序列分析发现cecD1和cecD2中分别包含568bp和377bp的内含子序列,它们有相同的5′和3′拼接位点,A+T含量分别为59.7%和69.8%,符合大多数真核生物内含子高A+T含量的特征。
译  名：
Cloning and sequence analysis of two cecropin D genes from the common cutworm,Spodoptera litura (Lepidoptera: Noctuidae)
作  者：
CHEN Wei-Chun~ 1,2 , SONG Jie~3, PANG Yi~ 1, (1. State Key Laboratory for Biocontrol, Institute of Entomology, Sun Yat-Sen University, Guangzhou 510275, China; 2. Insititute of Biochemistry and Molecular Biology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China; 3. Department of Parasitology, Guangdong Medical College, Zhanjiang, Guangdong 524023, China)
关键词：
Spodoptera litura; antibacterial peptide; cecropin; intron; gene structure
摘  要：
Cecropins are a family of antibacterial peptides synthesized in insects as a response to a bacterial infection. Two primers were designed according to cecropin B gene from Spodoptera litura and were used to PCR. As the result, two new cecropin genes, named cecD1 and cecD2, were obtained, which encoded identical preprocecropin D. There was 2 amino acid residues difference between the mature peptide of preprocecropin D and that of cecropin B from S. litura. Two introns, 568 bp and 377 bp long, were found in cecD1 and cecD2, respectively, both having the same 5′ and 3′splicing sites. The A+T content was 59.7% in cecD1 intron and 69.8% in cecD2 intron, conforming to the high A+T content characteristics of most eukaryotic introns.

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