Antioxidant activity in vitro of Lactobacillus plantarum NJAU-01

CHEN Lei;LIU Rui;GE Panwei;YANG Bo;WU Guoyue;WU Mangang;YU Hai;GE Qingfeng;College of Food Science and Engineering, Yangzhou University;

Lactobacillus plantarum NJAU-01;;antioxidant activity in vitro;;electrochemical assay;;macrophage RAW264.7

The objective of this study was to more comprehensively and objectively evaluate the antioxidant activity of Lactobacillus plantarum NJAU-01. In this paper, commercialized L.rhamnosus LGG and L.plantarum JMZ-12 were used as positive and negative controls, respectively. Free radical scavenging ability in vitro of lactic acid bacteria was compared. Moreover, electrochemical assay was used to rapidly assess ability of lactic acid bacteria to alleviate oxidative damage of macrophages RAW264.7 induced by H_2O_2. The results showed that DPPH free radical scavenging rate, hydroxyl radical scavenging rate, superoxide anion radical scavenging rate and reducing power of 10~9 CFU·L~(-1) NJAU-01 cell-free extracts were 24.20%, 29.03%, 18.21% and 40.13 μmol·L~(-1) L-cysteine, respectively, and its antioxidant capacity in vitro was comparable to the positive control LGG. Electrochemical assay results displayed that RAW264.7 after incubation with NJAU-01 cell-free extract had the most antioxidant capacity and the lowest intracellular reactive oxygen species. Our data suggest that NJAU-01 cell-free extract exhibited the most antioxidant capacity. It can be used as a research basis for further analysis of the antioxidant mechanism of lactic acid bacteria.