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Position: Home > Articles > 拉萨裸裂尻鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析 Acta Agriculturae Shanghai 2019 (3) 105-109

拉萨裸裂尻鱼5种组织乳酸脱氢酶和苹果酸脱氢酶的电泳分析

作  者:
张涛;陈建武;何力
单  位:
农业部水产品质量安全风险评估实验室(武汉)农业部淡水鱼类种质监督检验测试中心中国水产科学研究院长江水产研究所
关键词:
拉萨裸裂尻鱼;乳酸脱氢酶;苹果酸脱氢酶;组织特异性
摘  要:
为了解拉萨裸裂尻鱼的生化遗传特性,采用聚丙烯胺凝胶垂直板电泳分析了拉萨裸裂尻鱼5种组织(心脏、眼睛、肌肉、肝脏和肾脏)的乳酸脱氢酶(LDH)和苹果酸脱氢酶(MDH),并比较了拉萨裸裂尻鱼和黄河裸裂尻鱼乳酸脱氢酶的差异.结果表明:拉萨裸裂尻鱼的LDH同工酶和MDH同工酶具有组织特异性和个体差异性,所测样本鱼心脏、眼睛、肌肉、肝脏和肾脏中的LDH酶带数分别为12条、12-13条、14-15条、11-13条和10条; MDH酶带数分别为1条、1-3条、1条、1-3条和5条,拉萨裸裂尻鱼的MDH分为上清液型(s-MDH)和线粒体型(m-MDH).
作  者:
ZHANG Tao;CHEN Jian-wu;HE Li;Laboratory of Quality & Safety Risk Assessment for Aquatic Products(Wuhan) ,Ministry of Agriculture;Freshwater Fish Germplasm Quality Supervision and Testing Center,Ministry of Agriculture;Yangtze River Fisheries Research Institute,Chinese Academy of Fishery Sciences;
关键词:
Schizopygopsis younghusbandi;;LDH isozymes;;MDH isozymes;;Tissue specificity
摘  要:
By using the vertical polyacrylamide gel electrophoresis,lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isozymes from 5 tissues (heart,eye,muscle,liver and kidney) were preliminarily studied so as to learn about the biochemical heredity characteristics of Schizopygopsis younghusbandi. Meanwhile comparations of LDH isozymes between Schizopygopsis younghusbandi and Schizopygopsis pulzovi were studied.The results showed that: LDH isozymes and MDH isozymes showed tissue specificity and individual variation. The band numbers of LDH isozymes from heart,eye,muscle,liver and kidney were 12,12—13,14—15,11—13 and ten respectively,and MDH isozymes 1,1—3,1,1—3 and 5. MDH isozymes in Schizopygopsis younghusbandi were divided into supernate type and mitochondria type.

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