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当前位置: 首页 > 文章 > 小麦LRR类抗病相关基因及其cDNA 3′末端的克隆与分析 植物病理学报 2007,37 (3) 317-320
Position: Home > Articles > Cloning and analysis of LRR resistance related gene and its 3′cDNA end from wheat Acta Phytopathologica Sinica 2007,37 (3) 317-320

小麦LRR类抗病相关基因及其cDNA 3′末端的克隆与分析

作  者：
王海燕;杨文香;张汀;刘大群
单  位：
河北省农作物病虫害生物防治工程技术研究中心
摘  要：
Degenerated primer was designed based on the amino acid conserved regions of the reported plant disease resistance gene which encode leucine-rich repeats (LRR). A resistance gene analog (RGA) named LRR1 was obtained from the RNA of TcLr35 carrying Lr35 gene conferring resistance against wheat leaf rust by reverse transcription-polymerase chain reaction (RT-PCR). The fragment had an open reading frame (ORF) and the nucleotide sequence containing 215 bp in length that coded 58 amino acids. 3′RACE was carried out with gene specific primers designed based on LRR1 and 3′universal primer provided in the kit. An amplified fragment of 567 bp in length which overlapped with the known LRR1 sequence by 184 bp was obtained. Finally, a fragment of 598 bp containing the sequence of LRR1 except the 5′cDNA end was obtained.
译  名：
Cloning and analysis of LRR resistance related gene and its 3′cDNA end from wheat
作  者：
WANG Hai-yan, YANG Wen-xiang, ZHANG Ting, LIU Da-qun (Biological Control Center of Plant Pathogens and Plant Pests of Hebei Province, College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China)
关键词：
wheat leaf rust resistance gene; homology sequence; LRR; RACE
摘  要：
Degenerated primer was designed based on the amino acid conserved regions of the reported plant disease resistance gene which encode leucine-rich repeats (LRR). A resistance gene analog (RGA) named LRR1 was obtained from the RNA of TcLr35 carrying Lr35 gene conferring resistance against wheat leaf rust by reverse transcription-polymerase chain reaction (RT-PCR). The fragment had an open reading frame (ORF) and the nucleotide sequence containing 215 bp in length that coded 58 amino acids. 3′RACE was carried out with gene specific primers designed based on LRR1 and 3′universal primer provided in the kit. An amplified fragment of 567 bp in length which overlapped with the known LRR1 sequence by 184 bp was obtained. Finally, a fragment of 598 bp containing the sequence of LRR1 except the 5′cDNA end was obtained.

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