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Chinese Journal of Applied Entomology
2012,49
(3)
94-101
绿盲蝽取食诱导后棉花叶片内防御酶活力与基因表达量的变化
作 者：
毛红;杨宇晖;郭晨茜;张青文;刘小侠
单 位：
中国农业大学农学与生物技术学院
关键词：
绿盲蝽取食;外源水杨酸;外源茉莉酸甲酯;防御酶活性;基因表达
摘 要：
本研究以抗性棉皖棉小黄花为材料,室内条件下测定绿盲蝽Apolygus lucorum(Mayer-Dür)取食、机械损伤、外源水杨酸和外源茉莉酸甲酯诱导处理后棉叶中苯丙氨酸解氨酶基因(pal)、过氧化物酶基因(pod)和过氧化氢酶基因(cat)的相对表达量,并以健康植株为对照。结果表明:绿盲蝽取食诱导与外源信号物质诱导相似,苯丙氨酸解氨酶(PAL)和过氧化物酶(POD)活性升高,过氧化氢酶(CAT)活性降低;PAL、POD和CAT活力变化与本文中选取的pal、pod和cat基因表达量变化趋势存在差异。本研究说明,绿盲蝽取食既激活了水杨酸介导的防御信号转导途径,也激活了茉莉酸介导的信号转导途径;PAL、POD和CAT 3种酶活力不完全由本文中选取的pal、pod和cat 3个基因所调控。
译 名：
Changes in defensive enzyme activity and defensive enzyme gene expression in cotton leaves after feeding by Apolygus lucorum
作 者：
MAO Hong YANG Yu-Hui GUO Chen-Xi ZHANG Qing-Wen LIU Xiao-Xia(College of Agriculture and Biotechnology,China Agricultural University,Beijing 100193,China)
关键词：
Apolygus lucorum feeding,exogenous salicylic acid,exogenous methyl jasmonate,defense enzyme activity,gene expression
摘 要：
Active changes in some defensive enzymes(phenylalanine aminolyase,peroxidase and catalase),and the relative expression of the corresponding defensive genes(the phenylalanine aminolyase gene,peroxidase gene and catalase gene),were evaluated in leaves of the resistant cotton variety Wanmian xiaohuanghua in response to feeding by Apolygus lucorum(Mayer-Dür) and the application of exogenous salicylic acid and methyl jasmonate,under laboratory conditions.The results indicate that PAL and POD activity increased and CAT activity were decreased by A.lucorum feeding activity and the two chemical treatments.There were differences between changes in PAL,POD and CAT activity and those in pal,pod and cat gene expression.These results suggest that A.lucorum feeding activates the salicylic and jasmonate acid signaling pathways,and that PAL,POD and CAT activity are not completely regulated by the pal,pod and cat genes.
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