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农业科学学报 (英文)
2020,19
(6)
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Journal of Integrative Agriculture
2020,19
(6)
Characteristics and roles of cytochrome b5 in cytochrome P450-mediated oxidative reactions in Locusta migratoria
作 者：
Liu Jiao;Zhang Xue-yao;Wu Hai-hua;Ma Wen;Zhu Wen-ya;Zhu, Kun-Yan;Ma En-bo;Zhang Jian-zhe
单 位：
Kansas State Univ, Dept Entomol, Manhattan, KS 66506 USA;Shanxi Acad Agr Sci, Inst Plant Protect, Taiyuan 030031, Peoples R China;Shanxi Univ, Modern Res Ctr Tradit Chinese Med, Taiyuan 030006, Peoples R China;Shanxi Univ, Inst Appl Biol, Taiyuan 030006, Peoples R China;Shanxi Univ, Coll Life Sci, Taiyuan 030006, Peoples R China
关键词：
cytochrome b(5);cytochrome P450;cytochrome P450 reductase;Locusta migratoria;RNA interference
摘 要：
Cytochrome b(5) (Cyt-b(5)) is a small heme protein and known to be involved in a wide range of biochemical transformations, including cytochrome P450 monooxygenase (CYP)-mediated metabolism of endogenous and exogenous compounds. Studies on Cyt-b(5) are more concentrated in mammals, but are relatively rare in insects. The characteristics and function of Cyt-b(5) from Locusta migratoria have not been described yet. We sequenced the full-length cDNA sequence of Cyt-b(5) from L. migratoria (LmCyt-b(5)) by reverse transcription-PCR (RT-PCR) based on locust transcriptome database. The phylogenetic analysis showed that LmCyt-b(5) was closely related to the Cyt-b(5) from Blattodea. LmCyt-b(5) was highly expressed in ovary, Malpighian tubules, midgut, gastric caeca, and fat bodies. Silencing of LmCyt-b(5) had no effect on the susceptibility of L. migratoria to four different insecticides. Suppression of LmCyt-b(5) or silencing of both LmCyt-b(5) and LmCPR did not significantly change the total CYP activity toward the substrate 7-ethoxycoumarin (7-EC). However, coexpression of LmCYP6FD1 with LmCPR and LmCyt-b(5) together in Sf9 cells by using Bac-to-Bac baculovirus expression system significantly increased the catalytic activity of LmCYP6FD1 toward 7-EC as compared with the coexpression of LmCYP6FD1 with cytochrome P450 reductase (LmCPR) or LmCyt-b(5) separately. These results suggest that LmCyt-b(5) plays an important role in the catalytic reaction of LmCYP6FD1 toward 7-EC in our in vitro experiments. Further study is needed to clarify the role of LmCyt-b(5) in CYP-mediated catalytic reactions in L. migratoria.
