Huiling Wang;Xiao­hong Zhang;Xiaosu Zhou;Bing Wang;Xiaomin Wan

Capital Medical University, Beijing 100069,Chin;Capital Medical University, Beijing 100069,China

neurons;t10;β1;the hippocampus;42;mo

[Objective]To observe the preventive and therapeutic effects of T10 on A β1-42 oligomer mediated Alzheimer's Disease(AD) and its mechanism.[Method]The microglia cells were pre-treated with 10-11-10-8 mol/L T10 for 12 h before 10 μmol/L A β1-42 was added in,and incubated for 12 hours together.The content of TNF-α and IL-1β in supernatant were determined by ELISA.The conditioned medium was collected and was added with the hippocampus neurons treating for 24 hours.The survival rate of the neurons was detected by MTT.[Result]T10 could significantly decrease the release of TNF-and IL-1β induced by A β1-42 from microglia in vitro and raise the survival rate of the hippocampus neurons.[Conclusion]T10 could protect the hippocampus neurons through inhibiting the activation of microglia.