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Position: Home > Articles > A potential hyphal fusion protein complex with an important role in development and virulence interacts with autophagy-related proteins in Fusarium pseudograminearum Journal of Integrative Agriculture 2024,23 (12)

A potential hyphal fusion protein complex with an important role in development and virulence interacts with autophagy-related proteins in Fusarium pseudograminearum

作者：

Chen, Linlin;Shan, Yixuan;Dong, Zaifang;Zhang, Yake;Peng, Mengya;Yuan, Hongxia;Shi, Yan;Li, Honglian;Xing, Xiaoping

单位：

Natl Key Lab Wheat & Maize Crop Sci, Zhengzhou 450000, Peoples R China;Henan Agr Univ, Coll Plant Protect, Zhengzhou 450000, Peoples R China

关键词：

Fusarium pseudograminearum;hyphal fusion;FpHam-2;virulence;autophagy-related proteins

摘要：

Hyphal fusion (anastomosis) is a common process serving many important functions at various developmental stages in the life cycle of ascomycetous fungi. However, the biological roles and molecular mechanisms in plant pathogenic fungi were widely unknown. In this study, a hyphal fusion protein FpHam-2 was screened from a T-DNA insertion mutant library of Fusarium pseudograminearum, and FpHam-2 interacts with another 2 hyphal fusion protein homologues FpHam-3 and FpHam-4. Each of these 3 genes deletion mutant revealed in similar defective phenotypes compared with the WT and complemented strains, including reduction in growth rate, defects in hyphal fusion and conidiation, more sensitive for cell membrane, cell wall and oxidative stress responses, and decreased in virulence. The yeast two-hybrid assay was used to identify that FpHam-2 interacts with 3 autophagy-related proteins, including FpAtg3, FpAtg28 and FpAtg33. Furthermore, FpHam-2-deletion mutant showed decreased accumulation of autophagic bodies in hypha. In conclusion, FpHam-2, FpHam-3 and FpHam-4 have an essential role for hyphal fusion and regulating the growth, conidiation and virulence in F. pseudograminearum.

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A potential hyphal fusion protein complex with an important role in development and virulence interacts with autophagy-related proteins in Fusarium pseudograminearum

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