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Position: Home > Articles > SRAP Analysis of 15 Provenances of Pinus contorta Journal of Anhui Agricultural Sciences 2017,45 (12) 139-140+156

不同小干松种源的SRAP分析

作  者:
王骞春
单  位:
辽宁省林业科学研究院
关键词:
小干松;种源;SRAP
摘  要:
[目的]研究小干松种源遗传多样性,为小干松引种、育种工作提供基础资料。[方法]利用SRAP荧光标记技术对引种的15个小干松种源进行SRAP分析。[结果]9对荧光标记的SRAP引物共产生1 075个条带,多态性位点953个,平均多态性为88.7%,每对引物扩增条带75~146条,平均每对引物扩增条带119条;通过UPGMA软件对15个种源进行遗传聚类分析,15个种源的相似系数在0.761 3~0.876 0,以遗传相似系数0.850 0为阈值可将15个种源分为4个群体,同一地理来源的种源有一定的遗传差异,但大体呈现按地理来源聚类的趋势。[结论]15个小干松种源具有较高的基因多态性,遗传多样性丰富,小干松变异类型与地理分布有关。
译  名:
SRAP Analysis of 15 Provenances of Pinus contorta
作  者:
WANG Qian-chun;Liaoning Academy of Forestry Science;
关键词:
Pinus contorta;;Provenances;;SRAP
摘  要:
[Objective] To study the gentic diversity of Pinus contorta provenance for laying the foundation for introduction of P. contorta breeding. [Method] Using SRAP fluorescence technique to analyze 15 provenances of P. contorta. [Result]9 SRAP primers for fluorescent labeling produced a total of 1 075 bands,953 polymorphic loci,the average polymorphism was 88. 7%. Each primer amplified bands were from 75 to 146,an average of 119 bands. The similarity coefficients of 15 provenances were 0. 761 3-0. 876 0 by UPGMA software. Taking the genetic similarity coefficient of 0. 850 0 as threshold,15 provenances were divided into 4 clusters,the provenances with the same geographic origin had some genetic differences,but generally showed a trend of clustering according to geographic origin. [Conclusion]15 provenances of P. contorta had high genetic diversity,abundant genetic diversity,the variation types were related to geographic distribution.

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