Position: Home > Articles > Cloning and Expression of Gene Encoding Maltoologosyl Trehalose Synthase from Corynebacterium glutamicum
Journal of Agricultural Science and Technology
2009,11
(1)
72-76
谷氨酸棒杆菌麦芽寡糖基海藻糖合成酶基因的克隆与表达
作 者：
张文德;乔宇;丁宏标
单 位：
中国农业科学院饲料研究所
关键词：
麦芽寡糖基海藻糖合成酶;麦芽糊精;谷氨酸棒杆菌;重组表达
摘 要：
采用PCR方法从谷氨酸棒杆菌(Corynebacterium glutamicum)基因组中分离得到2.4 kb的麦芽寡糖基海藻糖合成酶基因(TreY),将其插入原核表达载体pET-30a中,转化大肠杆菌BL21(DE3)pLysS获得重组基因工程菌。经IPTG诱导表达得到约90 kDa的目的蛋白,可溶性蛋白占细胞总蛋白的45.3%。重组酶作用于麦芽糊精,可使其DE值降低,得到麦芽寡糖基海藻糖的混合物。
译 名：
Cloning and Expression of Gene Encoding Maltoologosyl Trehalose Synthase from Corynebacterium glutamicum
作 者：
ZHANG Wen-de,QIAO Yu,DING Hong-biao(Feed Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China)
关键词：
maltoologosyl trehalose synthase;malt dextrin;Corynebacterium glutamicum;recombinant expression
摘 要：
A 2.4 kb DNA fragment encoding maltooligosyl trehalose synthase was cloned from Corynebacterium glutamicum by PCR.It was inserted into prokaryotic expression vector pET-30a and then was introduced into the host Escherichia coli BL21(DE3) pLysS.A high yield of the recombinant enzyme at a molecular weight of 90 kDa was obtained by IPTG induction.The soluble recombinant enzyme accounted for 45.3% of the total cell proteins in supernatant.This recombinant enzyme was capable of decreasing the DE value of dextrin and producing a maltooligosyl trehalose mixture.
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