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Position: Home > Articles > Isolatwon and Identification of Newcastk Disease Virus and Estabishment of Resource platform in Shandong Province China Animal Husbandry & Veterinary Medicine 2009,36 (4) 157-160

山东省新城疫病毒的分离鉴定及资源平台的建立

作  者:
孙健;单虎
单  位:
青岛农业大学动物科技学院
关键词:
新城疫病毒;资源平台
摘  要:
作者根据山东省家禽养殖分布及新城疫病发报道进行病样采集,通过病毒分离培养、血凝试验及血凝抑制试验、电镜观察、病毒的最小致死剂量致死鸡胚的平均死亡时间和1日龄雏鸡脑内接种致病指数测定初步判定和描述新城疫病毒(newcastle disease virus,NDV)的生物学特性,以RT-PCR试验进行最终鉴定,共分离到60株毒株,按照兽医微生物资源描述规范的框架和数据录入要求,完成病毒资源标准化整合,并录入中国兽医微生物资源信息网的数据库,建立了山东省新城疫病毒资源平台。
译  名:
Isolatwon and Identification of Newcastk Disease Virus and Estabishment of Resource platform in Shandong Province
作  者:
SUN Jian,SHAN Hu(Animal Science College,Qingdao Agriculture University,Qingdao 266109)
关键词:
newcastle disease virus;resource platform
摘  要:
This study collected virus samples according to the distributing of poultry feeding and report news of newcastle disease in Shandong province.Biologic characters of NDV were identified by HA-HI,MDT,ICPI,electron microscope,and RT-PCR after virus samples were cultured.Sixty viruses were separated successfully,described standardizing according to the demand of veterinary resource platform,and submitted to the database of Chinese veterinary resource platform.Therefore,the resource platform of Newcastle disease virus in Shandong province was achieved.

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