中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 胡杨未减数2n花粉形成的细胞学机制 南京林业大学学报(自然科学版) 2015 (6) 40-44
Position: Home > Articles > Cytological mechanism of unreduced 2n pollen formation in Populus euphratica Journal of Nanjing Forestry University(Natural Sciences Edition) 2015 (6) 40-44

胡杨未减数2n花粉形成的细胞学机制

作  者：
赵晨光;李燕杰;张平冬;康向阳
单  位：
林木育种国家工程实验室林木花卉遗传育种教育部重点实验室北京林业大学
关键词：
胡杨;花粉母细胞;减数分裂;微管骨架;胞质分裂;未减数2n花粉
摘  要：
为了研究花粉母细胞减数分裂染色体的异常行为以及胞质分裂过程中微管骨架变化,利用液氮冷冻压片法和间接免疫荧光结合DAPI染色法,对胡杨花粉母细胞减数分裂过程中染色体行为和微管骨架进行了观察。结果表明:1胡杨花粉母细胞减数分裂前期Ⅰ同源染色体联会结合松散,在终变期可以观察到单价体;中期Ⅱ存在平行纺锤体、三极纺锤体以及融合纺锤体;后期Ⅰ以及后期Ⅱ可观察到落后染色体。2三极纺锤体以及融合纺锤体与未减数2n花粉比率呈正相关,说明三极纺锤体和融合纺锤体会导致FDR(first division restitution)型2n花粉的产生。3前期Ⅱ存在胞质分裂提前现象,可导致SDR(second division restitution)型2n花粉形成。4后期Ⅱ纺锤体微管缺失致使姊妹染色体单体分离后不移向两极,保留在同一子核内,这可能是形成SDR型2n花粉的另一种机制。
译  名：
Cytological mechanism of unreduced 2n pollen formation in Populus euphratica
作  者：
ZHAO Chenguang;LI Yanjie;ZHANG Pingdong;KANG Xiangyang;National Engineering Laboratory for Tree Breeding, Key Laboratory for Genetic and Breeding in Forest Trees and Ornamental Plants, Ministry of Education,Beijing Forestry University;
关键词：
Populus euphratica;;pollen mother cell;;meiosis;;microtubule cytoskeleton;;cytokinesis;;unreduced 2n pollen
摘  要：
In order to analyze abnormal behaviors of chromosomes during meiosis and abnormal organization of microtu-bule during meiosis and cytokinesis, and find a new method to illustrate unreduced 2n pollen formation, indirect immu-nofluorescence microscopy with DAPI staining was conducted to observe the microtubule cytoskeleton pattern and chro-mosomes behavior during meiosis and cytokinesis inPopulus euphratica. The results indicated that: 1 During the pro-phase stage of meiosis, the paired homologous chromosomes combined, loosely, with univalent at diakinesis. Parallelspindle, tripolar spindle and fused spindle could also been found, at metaphase Ⅱ Lagging chromosomes could be ob-served at anaphaseⅠand anaphaseⅡ. 2 Positive correlations between frequency of 2n pollen and rate of tripolar spindleand fused spindle were found, which suggested that tripolar spindle and fused spindle could result in FDR-typed 2n pol-len formation. 3 Prematurely cytokinesis was observed at prophaseⅡ, which resulted in SDR-typed 2n pollen formationinPopulus euphratica. 4 Due to lack of spindle microtubule in one daughter cell at anaphaseⅡ, the chromatids do notmigrated to the poles instead of retaining in the daughter cell and forming a SDR-typed 2n pollen.

相似文章

导出引用
计量
文章访问数: 4
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46