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当前位置: 首页 > 文章 > 高效除臭菌的筛选及其降解特性研究 农业环境科学学报 2004,23 (02) 300-303
Position: Home > Articles > Optimized Cultivation of Highly- Efficient Deodorizing Bacterial Consortia and their Degradation Ability towards Pyrene Journal of Agro-Environment Science 2004,23 (02) 300-303

高效除臭菌的筛选及其降解特性研究

作  者：
余海晨;张清敏;侯树宇;张杨;白晔
单  位：
南开大学环境科学与工程学院
关键词：
恶臭;DMSO;H2S;半胱氨酸;除臭菌
摘  要：
采用室内培养实验方法,讨论了从垃圾渗滤液中驯化分离出能够耐受并利用二甲基亚砜(DMSO)作为营养物质的有效菌群,并以含巯基的半胱氨酸模拟自然界中恶臭物质H2S的产生和H2S被氧化的过程。研究结果表明,在半胱氨酸浓度为0.1mg·mL-1时,能够在培养的第2d大部分被该菌群分解成H2S,在培养的第5d所产生的H2S有82%被氧化为高价态的硫化物,达到了很好的除臭效果。经过多次平板划线筛选,分离出8株有效菌C1～C8,经生理生化性能测定,初步鉴定这8株均为化能异养的芽孢杆菌,好氧或兼性厌氧。
译  名：
Optimized Cultivation of Highly- Efficient Deodorizing Bacterial Consortia and their Degradation Ability towards Pyrene
作  者：
YU Hai chen, ZHANG Qing min, HOU Shu yu, ZHANG Yang, BAI Ye(College of Environmental Science and Engineering, Nankai University, Tianjin 300071,China)
关键词：
malodor; DMSO; H_2S; cysteine; deodorizing bacteria
摘  要：
Highly- efficient deodorizing bacterial consortia capable of tolerance and utilizing high concentration of dimethyl sulfoxide( DMSO) were isolated and cultivation was optimized from a landfill leachate. We studied degradation process and used products of cysteine( Cys) as source of H2S and simulated generation and oxidation process of H2S as a main malodorous gas from nature in the present study. The results showed that maximum conversion rate of Cys could reach up to 78.75% at the second day of cultivation under a condition that the concentration of Cys was 0.1 mg· mL- 1, and further oxidation rate of H2S could reach up to 82% at the fifth day of cultivation, exhibiting a highly- efficient deodorization. Eight bacterial consortia C1～ C8 were isolated in our repetitious optimized cultivations process. All of the eight available strains were proved to be heterotrophic bacillus, either aerobes or facultative aerobes.

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