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当前位置: 首页 > 文章 > 鲈细菌性类结节病的病原及血液病理研究 水产学报 2004,28 (6) 96-101
Position: Home > Articles > Pathogenic and hemopathological studies on the bacterial pseudotuberculosis of Lateolabrax japonicus Journal of Fisheries of China 2004,28 (6) 96-101

鲈细菌性类结节病的病原及血液病理研究

作  者：
金珊;王国良;赵青松;陈惠群;陈寅儿
单  位：
宁波大学海洋与水产系
关键词：
鲈;类结节病;嗜水气单胞菌
摘  要：
2002年5月从患细菌性类结节病的濒死鲈体内分离到一株细菌02517 1,经人工感染试验及API20E系统鉴定,认为该病原菌为嗜水气单胞菌(Aeromonashydrophila)。血液生理生化指标检测显示,与健康鱼相比,患病鱼的红细胞、白细胞、血糖、蛋白质、尿素、胆固醇等显著下降,而谷丙转氨酶ALT、谷草转氨酶AST、乳酸脱氢酶LDH等血清酶类活性增强,说明经嗜水气单胞菌感染后,鲈在营养物质代谢、抗病力、消化、呼吸、排泄等方面的生理功能受到严重损害。药敏实验结果表明:复方新诺明、卡那霉素、庆大霉素、氟哌酸等4种化学疗剂及地榆、乌梅、木瓜、五味子等4种中草药对该种病原菌有较强的抑菌作用。
译  名：
Pathogenic and hemopathological studies on the bacterial pseudotuberculosis of Lateolabrax japonicus
作  者：
JIN Shan, WANG Guo-liang, ZHAO Qing-song, CHEN Hui-qun, CHEN Yin-er (Department of Oceanography and Fisheries, Ningbo University, Ningbo315211, China)
关键词：
Lateolabrax japonicus; pseudotuberculosis; Aeromonas hydrophila
摘  要：
One strain of bacteria 02517-1 was isolated from Lateolabrax japonicus with the bacterial pseudotuberculosis in May 2002. After the pure culture, the bacteria were used to infect the health Lateolabrax japonicus in three ways: abdominal cavity injection (0.3mL/nos), wound soaking, and soaking. The concentration of bacterial suspension was determined by both McF and live bacterial counting technique. The physiological saline was substitute for bacterial suspension in the control. The concentration of soaking was 1.52×10~4 Cells·mL~(-1). The result showed that the strain of bacteria 02517-1 had powerful pathogenicity and the mortality of healthy fish was 100%. According to Bergey's, manual of systematic bacteriology and API 20E system indentification, the pathogen of Lateolabrax japonicus was identified as Aeromonas hydrophila. Serums were extracted from the blood of both diseased fish and healthy fish, and the blood biochemical and physiological indices were determined by Olympus biochemical automatic instrument. The results showed that, compared with healthy ones, the number of red blood cells, white blood cells, glucose,total plasma protein, ureanitrogen and cholesterin in diseased fish all decreased remarkably, while serum enzymes of ALT,AST, LDH increased. These results indicated that many important physiological functions such as digestion, breath, excretion, metabolism and disease resistance were seriously impaired after Lateolabrax japonicus infection. The result of antibiotic sensitivity test showed that four chemotherapeutants (compound sulfamethoxazole, kanamycin, gentamycinum, norfloxacin) and four Chinese herbal medicines (sanguisorba, armeniaca, chaenomeles, schisandra) were most effective against the pathogenic bacteria.

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