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当前位置: 首页 > 文章 > 罗非鱼致病性海豚链球菌药敏及中草药提取液体外抑菌试验 广东农业科学 2010,37 (9) 162-165
Position: Home > Articles > Studies on antibacterial effects of antibiotic drugs and Chinese medicinal herbs to the pathogen of Streptococcus iniae disease from tilapia in Vitro Guangdong Agricultural Sciences 2010,37 (9) 162-165

罗非鱼致病性海豚链球菌药敏及中草药提取液体外抑菌试验

作  者：
张彬;黄婷;陈福艳;梁万文;余晓丽;雷爱莹;陈明
单  位：
广西水产研究所鱼病研究室
关键词：
中草药;海豚链球菌;药敏试验;抑菌
摘  要：
采用琼脂扩散法和二倍稀释法测定了28种抗生素和10味中草药对一株罗非鱼致病性海豚链球菌(Streptococcus iniae)的抑菌作用。结果表明:28种抗菌类药物中,病原菌对头孢克洛、克拉霉素、头孢噻吩、先锋必、菌必治、头孢拉定、新生霉素、先锋Ⅴ、罗红霉素、头孢呋肟、头孢氨苄11种药物敏感,对卡那霉素、氨曲南、头孢克肟、林可霉素4种药物表现耐药性。10种中草药水提取物对海豚链球菌均有不同程度的抑菌作用,其中黄连水提物的抑菌作用最佳,其对海豚链球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)分别为3.75mg/mL和7.50mg/mL,抑菌圈平均直径达20mm以上;金银花、黄芩、大黄、连翘、黄柏有一定的抑菌作用,MIC为7.50~15.0mg/mL,抑菌圈平均直径均在10mm以上;而大青叶、穿心莲、蒲公英、龙胆草的抑菌作用不明显或无抑菌效果。
译  名：
Studies on antibacterial effects of antibiotic drugs and Chinese medicinal herbs to the pathogen of Streptococcus iniae disease from tilapia in Vitro
作  者：
ZHANG Bin,HUANG Ting,CHEN Fu-yan,LIANG Wan-wen,YU Xiao-li,LEI Ai-ying,CHEN Ming (Lab of Fish Disease,Guangxi Fishery Research Institute,Nanning 530021,China)
关键词：
Chinese herbal medicine; Streptococcus iniae; drug sensitive test; antibacterial
摘  要：
The research aimed to investigate the antibacterial effects of 28 antibiotics and 10 Chinese herbal medicines to the pathogen of Streptococcus iniae disease from tilapia by disc agar diffusion and microdilution procedure. The results showed that Streptococcus iniae were highly sensitive to Cefaclor, Clarithromycin, Cefalotin, Cefobid, Ceftriaxone, Cefradine, Albamycin, Cephaloridine Ⅴ, Roxithromycin, Cefuroxime and Cephalexin, and which were resistant to Kanamycin, Aztreonam, Cefixime and Lyncomycin. In the meantime, the results showed that 10 Chinese herbal medicine water extracts had inhibitory effect on Streptococcus iniae to different degree. The antibacterial effect of Rhizoma Coptidis was better than the other nine kinds with the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) to Streptococcus iniae were 3.75 mg/mL and 7.50 mg/mL respectively, and which inhibitory circle exceeded 20 mm; Flos Lonicerae, Scutellaria baicalensis, Radix et Rhizoma Rhei, Forsythia suspense and Cortex Phellodendri chinesis had to some extent inhibition of the bacterium with MIC of 7.50～15.0 mg/mL, and which inhibitory circle of the five medicine extracts exceeded 10 mm, but Folium Isatidis, Herba Andrographitis, Herba Taraxaci and Radix Gentianae had no inhibition of the bacterium with MIC of more than 240 mg/mL.

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