Position: Home > Articles > The RNA extraction and detection of sperm cDNA quality in pig
Heilongjiang Animal Science and Veterinary Medicine
2014
(7)
67-69+215
猪精子RNA提取及cDNA质量检测
作 者：
高波;宋成义
单 位：
扬州大学动物科学与技术学院
关键词：
精子RNA;cDNA;猪
摘 要：
为了有效分离猪精子RNA,试验通过精子上游法获得纯净精子样本,采用改良TRIzol法提取精子总RNA,经核酸测定仪及琼脂糖凝胶电泳检测RNA的纯度和质量,RT-PCR检测相应体细胞标记基因E-Cadherin、CD4和c-kit。结果表明:研究建立的精子RNA提取法可获得高质量的RNA,浓度约为203 ng/μL,OD260/280值为1.99,并且无其他体细胞标记基因扩增产物,可用于猪精子mRNA的研究。
译 名：
The RNA extraction and detection of sperm cDNA quality in pig
作 者：
GAO Bo;SONG Cheng-yi;College of Animal Science and Technology,Yangzhou University;
关键词：
sperm RNA;;cDNA;;pig
摘 要：
To effectively isolate the sperm RNA in pig,the swim-up protocol was used to obtain the purified sperms. The total RNA from sperm was extracted using an improved Trizol method,and then the purity and quantity of RNA were determined using a nucleic acid tester and agarose gel electrophoresis. The RT-PCR was used to detect the E-Cadherin gene,CD4 gene and c-kit gene used as the marker genes corresponding to the somatic cells. The results showed that the established extraction method for sperm RNA could obtain high-quality RNA,its concentration was approximately 203 ng /μL,and the OD260 /280ratio was 1. 99. There were no other amplification products from the other marker genes of the somatic cells. The results indicate that the extraction method can be used to study sperm mRNA in pig.
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