中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 武汉肉类食品中大肠杆菌O157∶H7分离株stx亚型和毒力特征分析 食品科学 2014,35 (8) 94-98
Position: Home > Articles > stx Genotypes and Virulence Characteristics of E. coli O157:H7 Strains Isolated from Meat Products Commercialized in Wuhan FOOD SCIENCE 2014,35 (8) 94-98

武汉肉类食品中大肠杆菌O157∶H7分离株stx亚型和毒力特征分析

作  者：
郑冬冬;毕旺来;王宏勋;刘志国;丁洪波;李睿
单  位：
武汉轻工大学食品科学与工程学院;武汉轻工大学生物与制药工程学院
关键词：
大肠杆菌O157∶H7;志贺毒素;stx亚型;Vero细胞毒性
摘  要：
从22家市场销售的117份肉类食品中分离出4株大肠杆菌O157∶H7菌株,经PCR检测这4株菌的stx、hly、eae毒力基因均为阳性。采用聚合酶链式反应(polymerase chain reaction,PCR)法对这4株菌的stx亚型进行鉴定。3株菌同时携带stx1、stx2基因,且均为stx1a、stx2a亚型。菌株EC5.11仅携带stx2基因,但在所有的stx2分型PCR反应中都为阴性。用PCR扩增该菌株stx2基因全长并克隆测序,序列分析结果表明EC5.11志贺毒素基因为stx2c亚型。采用Vero细胞毒性实验检测这4株菌产志贺毒素的状况,结果显示这些菌株都具有一定的Vero细胞毒性。
译  名：
stx Genotypes and Virulence Characteristics of E. coli O157:H7 Strains Isolated from Meat Products Commercialized in Wuhan
作  者：
ZHENG Dong-dong;BI Wang-lai;WANG Hong-xun;LIU Zhi-guo;DING Hong-bo;LI Rui;College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University;College of Food Science and Engineering wuhan polytechnic university;Hubei Collaborative Innovation Center for Processing of Agricultural Products;
关键词：
E.coli O157:H7;;Shiga-toxin;;stx genotype;;Vero cytotoxicity
摘  要：
A total of 117 raw meat samples were collected from 22 markets in Wuhan, China and four E. coli O157:H7 strains were isolated from these meat samples, which were positive for stx, hly and eae genes. The stx variant genes were further subtyped by polymerase chain reaction(PCR) using sequence-specific primers targeting each stx variant. Three isolates were found to carry both stx1 and stx2 genes, and the prevalent stx genotypes were stx1a and stx2a. One isolate EC 5.11 was found to carry only stx2 gene but it could not be subtyped by PCR assay. The entire stx2 gene was then amplified from EC 5.11, cloned into a vector and sequenced. Sequencing data showed that the stx2 gene of EC5.11 was 100% identical to the published sequences of stx2c. The production of Shiga toxin from the four isolates was confirmed by Vero cell toxicity assay.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46