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Position: Home > Articles > Quick-propagation technique by tissue culture for Populus alba×berolinensis Journal of Jilin Forestry Science and Technology 2003,32 (3) 10-13

银中杨组培快繁技术的研究

作  者:
章林;陈建军;陆志民;王筱娜;魏松燕;张忠辉;章红;刘玉明;章森
单  位:
吉林省林业科学研究院;吉林省辉南县林业局;梅河口市林业局
关键词:
银中杨;组织培养;培养基
摘  要:
本文对银中杨的组培快繁技术进行了研究,结果表明,取外植体带有侧芽的茎条切段进行组织培养,以ACM为基本培养基,附加不同量的6-BA与NAA进行分化诱导,可获得丛壮无根苗;以附加NAA诱导生根可获得再生植株。该技术扩繁速率符合工厂生产的要求。
译  名:
Quick-propagation technique by tissue culture for Populus alba×berolinensis
作  者:
ZHANG Lin1, CHEN Jian-jun1, LU Zhi-min1, WANG Xiao-na1,WEI Song-yan1, ZHANG Zhong-hui1, ZHANG Hong2, LIU Yu-ming3,ZHANG Sen2(1.Jilin Provincial Academy of Forestry Science, Changchun 130031, China; 2.Huinan Forestry Bureau of Jilin Province, Tonghua 135100, China; 3.Forestry Bureau of Meihekou City, Tonghua 135000, China)
关键词:
Populus alba×berolinensis; tissue culture; medium
摘  要:
Quick-propagation technique of Populus alba×berolinensis was studied in experiment. It shows that fasciculate rootless plants could be obtained under following conditions: Taking ACM as basal medium make tissue culture to twiglet (explants with lateral bud), also adding 6-BA and NAA at proper doses and inducing differentiation; and then bring regrowths by adding NAA to induce root initiation. This technique accords with industrial seedling in the rate of extended production.

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