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Position: Home > Articles > Genetic Diversity Analysis of Six Wild Populations of Fat Greenling Hexagrammos otakii by Mitochondrial DNA 12S rRNA Partial Sequence Chinese Journal of Fisheries 2017,30 (5) 7-12

大泷六线鱼6个野生群体遗传多样性的12S rRNA基因分析

作  者:
王伟;高伟峰;张赛赛;董安然;姜欣彤
单  位:
农业部北方海水增养殖重点实验室
关键词:
大泷六线鱼;野生群体;线粒体DNA;12S rRNA部分序列
摘  要:
本文利用PCR技术扩增了6个野生群体大泷六线鱼线粒体12S r RNA基因的部分序列。在获得的582bp碱基序列中,共检测到314个变异位点,占总位点数的53.95%。34尾个体共定义了16种单倍型,6个群体的单倍型多样性指数为0.40~1.00,核苷酸多样性指数为0.00071~0.10441。中性检验Fu's Fs值为1.19782(P<0.01)。分子变异分析表明:Fst=0.88326(P<0.01),群体间和群体内变异分别为88.33%和11.67%。不同个体间的遗传距离构建的NJ和MP系统树表明,东港和旅顺群体亲缘关系较近,构成一个分支,其他群体构成另一分支。
译  名:
Genetic Diversity Analysis of Six Wild Populations of Fat Greenling Hexagrammos otakii by Mitochondrial DNA 12S rRNA Partial Sequence
作  者:
WANG Wei;GAO Wei-feng;ZHANG Sai-sai;DONG An-ran;JIANG Xin-tong;Key Laboratory of Mariculture & Stock Enhancement in North China's Sea, Ministry of Agriculture, Dalian Ocean University;Tianjin Chen Hui Modern Technology Group Co., Ltd.;
关键词:
Hexagrammos otakii;;wild population;;mitochondrial DNA;;12S rRNA partial sequence
摘  要:
Nucleotide sequences of mitochondrial DNA 12 S rRNA partial sequence were amplified in six wild populations of fat greenling Hexagrammos otakii using PCR techniques. A total of 314 variable sites were detected among the aligned sequences of 582 bp with mutation rate of 53.95%. Sixteen haplotypes were identified from 34 individuals according to the determined sequences, and the six populations had haplotype diversity of 0.40 ~ 1.00, nucleotide diversity of 0.00071 ~ 0.10441 and Fu's Fs of 1.19782 in neutrality tests(P < 0.01). AMOVA analysis demonstrated that: there were Fst = 0.88326(P < 0.01), variance of 88.33% among six populations and variance of 11.67% within the six populations. The NJ and MP molecular phylogenetic trees constructed by the distances among different individuals revealed that the phylogenetic trees were all divided into two branches, one in Donggang and Lvshun populations and the others in the other populations.

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